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Gene Expression Data
Assay Details
Assay
Reference: J:173838 Manuylov NL, et al., Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol. 2011 May 15;353(2):229-41
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7782571
Gene symbol: Gata4
Gene name: GATA binding protein 4
Antibody: anti-Gata4 (N-terminal)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2A control: embryonic day 11.5 (more )
Note: Double labeled: red - Gata4; green - Pecam1 (assay MGI:7782572).
Structure Level Pattern Image Note
TS19: coelomic epithelium of gonad Present 2A Control
TS19: gonad Present 2A Control Expressed in the somatic cells.
Specimen 2A GATA4cKO: embryonic day 11.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Pecam1 (assay MGI:7782572).
Structure Level Pattern Image Note
TS19: gonad Present 2A cKO Reduced expression compared with the control.
TS19: coelomic epithelium of gonad Present 2A cKO Many coelomic cells retained expression.
Specimen 2B control: embryonic day 12.5 (more )
Note: Double labeled: red - Gata4; green - Pecam1 (assay MGI:7782572).
Structure Level Pattern Image Note
TS20: coelomic epithelium of testis Present 2B Control
TS20: testis Present 2B Control Expressed in the somatic cells.
Specimen 2B GATA4cKO: embryonic day 12.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Pecam1 (assay MGI:7782572).
Structure Level Pattern Image Note
TS20: coelomic epithelium of testis Present 2B cKO Many coelomic cells retained expression.
TS20: testis Weak 2B cKO Expressed in a few rare cells below the coelomic epithelium.
Specimen 8C GATA4cKO: embryonic day 12.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Zfpm2 (assay MGI:7783738).
Structure Level Pattern Image Note
TS20: coelomic epithelium of testis Present 8C Most cells in the coelomic domain retained expression.
Specimen S3B/C GATA4cKO: embryonic day 12.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Zfpm2 (assay MGI:7783738).
Structure Level Pattern Image Note
TS20: coelomic epithelium of testis Present S3B , S3C Residual staining in the coelomic area was seen.
TS20: testis Weak Regionally restricted S3B , S3C Only expressed in a few rare cells below the coelomic epithelium.
Specimen S3E/F FOG2cKO: embryonic day 14.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Gata4; green - Zfpm2 (assay MGI:7783738).
Structure Level Pattern Image Note
TS22: gonad Present S3E , S3F Continued to be expressed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory