Probe:	Mm-Zic1 (493121)
Probe preparation:	Antisense
Visualized with:	Chromogenic

Structure	Level	Pattern	Image	Note
TS17: cranial mesenchyme	Present	Regionally restricted	9L	At E10.5 expression was detected in the mesenchyme surrounding the brain, but it was already excluded from the mesenchyme above the eye.

Structure	Level	Pattern	Image	Note
TS19: cranial mesenchyme	Present	Regionally restricted	9O , 9M , 9N	At E11.5 expression was detected in the mesenchyme surrounding the brain, but it was already excluded from the mesenchyme above the eye.

Structure	Level	Pattern	Image	Note
TS22: cranial mesenchyme	Present	Regionally restricted	9P , 9S , 9Q , 9R	Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer. Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region, while strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
TS22: frontal bone primordium	Ambiguous	Regionally restricted	9P , 9S , 9R	Most of the developing frontal bone was devoid of staining.

Structure	Level	Pattern	Image	Note
TS24: cranial suture mesenchyme	Strong		9T , 9U	Strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
TS24: head mesenchyme	Present		9T , 9V , 9U , 9W	Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region. Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer.
TS24: frontal bone	Ambiguous		9W , 9V , 9T	Most of the frontal bone was devoid of expression.

Structure	Level	Pattern	Image	Note
TS26: cranial suture mesenchyme	Strong	Regionally restricted	9Y , 9X , 9Z , 9a	Strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
TS26: frontal bone	Present	Regionally restricted	9X , 9a , 9Z , 9Y	Most of the developing frontal bone was devoid of Zic1 mRNA, but some expression was detected at the apical end as the osteogenic front approached the interfrontal suture.

Structure	Level	Pattern	Image	Note
TS22: cranial mesenchyme	Present	Regionally restricted	S13J , S13L , S13I , S13K	Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer. Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region, while strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
TS22: parietal bone primordium	Present	Regionally restricted	S13L , S13I , S13K	The parietal bone showed negligible expression of Zic1 on the basal end, but had some expression on the lateral and apical parts.

Structure	Level	Pattern	Image	Note
TS24: cranial suture mesenchyme	Strong	Regionally restricted	S13P , S13O	Strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
TS24: head mesenchyme	Present	Regionally restricted	S13Q , S13R , S13O	Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region. Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer.
TS24: parietal bone	Present	Regionally restricted	S13R , S13Q , S13O	The parietal bone showed negligible expression of Zic1 on the basal end, but had some expression on the lateral and apical parts.

Structure	Level	Pattern	Image	Note
TS26: cranial suture mesenchyme	Strong	Regionally restricted	S13S , S13T	Strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
TS26: head mesenchyme	Present	Regionally restricted	S13S , S13U , S13V	Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region. Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer.
TS26: parietal bone	Present	Regionally restricted	S13U , S13S , S13V	The parietal bone showed negligible expression of Zic1 on the basal end, but had some expression on the lateral and apical parts.