Probe:	BMPER cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	4A	Expression was detected in the dorsal midline in the most rostral region of the telencephalon.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	4B , 4C	Expression was sharply restricted to midline in the cell layer adjacent to the telencephalic vesicle, corresponding to the lamina reuniens.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: migrating neural crest	migratory neural crest cell	Present	Regionally restricted	4D	At the levels of somites 8 to 12, a migrating population of branchial neural crest cells ventrolateral to the neural tube and dorsal to Rathke's pouch stained positive.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: aorta-gonad-mesonephros		Present	Regionally restricted	4G , 4E	Signal was observed in a symmetrical region ventrolateral to the dorsal aorta.
TS17: dorsal aorta	blood vessel endothelial cell	Present		4E , 4G	A ring-shaped layer of cells around the dorsal aorta, which are presumptive endothelial cells, was also stained.
TS17: trunk mesenchyme	mesenchymal cell	Present	Scattered	4E , 4G	Expression was detected in scattered adjacent mesenchymal or migrating cells.

Structure	Level	Pattern	Image	Note
TS15: tail	Weak	Regionally restricted		Weak dorsal midline signal was detected in the distal part of the tail.

Structure	Level	Pattern	Image	Note
TS15: yolk sac blood vessel	Present	Regionally restricted	5A	Expression was detected in a reticular staining pattern consistent with staining of the developing yolk sac vasculature.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: yolk sac blood vessel	blood vessel endothelial cell	Present	Regionally restricted	5B	Expression was detected in endothelial cells lining the inner layer of the vascular wall.