Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212179 Ranade SS, et al., Piezo1, a mechanically activated ion channel, is required for vascular development in mice. Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10347-52
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7778386
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	MEC 13.3
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

S2A Piezo^+/+ ©

Specimen S2A +/+: embryonic day 9.5 (more

)

Structure	Level	Image
TS15: blood vessel	Present	S2A Piezo^+/+
TS15: cardinal vein	Present	S2A Piezo^+/+
TS15: dorsal aorta	Present	S2A Piezo^+/+

Specimen S2A gt/gt: embryonic day 9.5; Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} (more

)

Structure	Level	Image	Note
TS15: blood vessel	Present	S2A Piezo^gt/gt	Expression in this mutant is the same as in controls.
TS15: cardinal vein	Present	S2A Piezo^gt/gt	Expression in this mutant is the same as in controls.
TS15: dorsal aorta	Present	S2A Piezo^gt/gt	Expression in this mutant is the same as in controls.

3A Piezo^+/+ ©

Specimen 3A +/+: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: yolk sac blood vessel	Present		3A Piezo^+/+

3A Piezo^gt/gt ©

Specimen 3A gt/gt: embryonic day 8.5; Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS13: yolk sac blood vessel	Present		3A Piezo^gt/gt	The organization of the early vascular plexus is significantly altered in this mutant compared to controls.

S5A Piezo^+/+ ©

Specimen S5A +/+: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S5A Piezo^+/+

Specimen S5A gt/gt: embryonic day 9.5; Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S5A Piezo^gt/gt	The vasculature is less defined and the number of major vessels is reduced relative to controls.

Specimen S5B E9.5 +/+: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: head blood vessel	Present		S5B E9.5 Piezo^+/+

Specimen S5B E9.5 gt/gt: embryonic day 9.5; Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS15: head blood vessel	Present		S5B E9.5 Piezo^gt/gt	This mutant is lacking long branches.

Specimen S5B E10.5 +/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: head blood vessel	Present		S5B E10.5 Piezo^+/+

Specimen S5B E10.5 gt/gt: embryonic day 10.5; Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS17: head blood vessel	Present		S5B E10.5 Piezo^gt/gt	This mutant has fewer large head vessels and irregular branching.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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