Gene Expression Data
Assay Details
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Assay
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Reference:
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J:175534
Sipe CW, et al., Kif3a regulates planar polarization of auditory hair cells through both ciliary and non-ciliary mechanisms. Development. 2011 Aug;138(16):3441-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7778315
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Gene symbol:
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Fzd3
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Gene name:
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frizzled class receptor 3
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Antibody:
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anti-frizzled 3
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
S2A:
embryonic day 17.5
(more  )
Note: Double labeled: red - Fzd3; green - phalloidin.
Specimen
S2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Fzd3; green - phalloidin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: organ of Corti |
auditory epithelial supporting cell |
Present |
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S2A
,
S2C
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Expression is enriched on the medial side of plasma membrane in hair cells and support cells.
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| TS25: organ of Corti |
auditory hair cell |
Present |
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S2A
,
S2C
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Expression is enriched on the medial side of plasma membrane in hair cells and support cells.
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Specimen
S2B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1+
Kif3atm2Gsn/Kif3atm2Gsn
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Double labeled: red - Fzd3; green - phalloidin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: organ of Corti |
auditory epithelial supporting cell |
Present |
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S2D
,
S2B
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In the basal region of this mutant protein localization was largely normal.
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| TS25: organ of Corti |
auditory hair cell |
Present |
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S2D
,
S2B
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In the basal region of this mutant protein localization was largely normal.
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Specimen
S2E:
embryonic day 17.5
(more )
Note: Double labeled: red - Fzd3; green - phalloidin.
Specimen
S2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Fzd3; green - phalloidin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: organ of Corti |
auditory epithelial supporting cell |
Present |
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S2G
,
S2E
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Expression is enriched on the medial side of plasma membrane in hair cells and support cells.
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| TS25: organ of Corti |
auditory hair cell |
Present |
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S2G
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S2E
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Expression is enriched on the medial side of plasma membrane in hair cells and support cells.
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Specimen
S2F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1+
Kif3atm2Gsn/Kif3atm2Gsn
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Double labeled: red - Fzd3; green - phalloidin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: organ of Corti |
auditory epithelial supporting cell |
Present |
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S2F
,
S2H
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Toward the apex in this mutant there is asymmetric membrane localization.
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| TS25: organ of Corti |
auditory hair cell |
Present |
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S2F
,
S2H
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Toward the apex in this mutant there is asymmetric membrane localization.
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Analysis Tools