Gene Expression Data
Assay Details
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Assay
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Reference:
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J:317249
Yin FT, et al., Caspr4 interaction with LNX2 modulates the proliferation and neuronal differentiation of mouse neural progenitor cells. Stem Cells Dev. 2015 Mar 1;24(5):640-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7768117
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Gene symbol:
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Cntnap4
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Gene name:
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contactin associated protein-like 4
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Antibody:
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anti-Caspr4
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1A:
embryonic day 14.0
(more  )
Note: Double labeled: green - Cntnap4; red - Nes (assay MGI:7768118).
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cntnap4; red - Nes (assay MGI:7768118).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex subventricular zone |
neural progenitor cell |
Present |
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1A Caspr4 inset
,
1A Caspr4/Nestin inset
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1A Caspr4
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1A Caspr4/Nestin
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Expression in Nes-expressing neural progenitor cells.
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Specimen
1B:
embryonic day 14.0
(more )
Note: Double labeled: red - Cntnap4; green - Sox2 (assay MGI:7768120).
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cntnap4; green - Sox2 (assay MGI:7768120).
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Specimen
4E:
embryonic day 14.0
(more )
Note: Double labeled: green - Cntnap4; red - Lnx2 (assay MGI:7768121).
Specimen
4E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cntnap4; red - Lnx2 (assay MGI:7768121).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex subventricular zone |
neural progenitor cell |
Present |
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4E Caspr4/LNX2
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4E Caspr4
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Expression colocalized with Lnx2 expression in neural progenitor cells.
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Specimen
S2A:
postnatal day 0
(more )
Note: Double labeled: red - Cntnap4; green - Nes (assay MGI:7768118).
Specimen
S2A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cntnap4; green - Nes (assay MGI:7768118).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: cerebral cortex subventricular zone |
neural progenitor cell |
Present |
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S2A Caspr4 inset
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S2A Caspr4
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S2A Caspr4/Nestin inset
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S2A Caspr4/Nestin
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Expression in Nes-expressing neural progenitor cells.
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Specimen
S2C:
postnatal adult
(more )
Note: Double labeled: red - Cntnap4; green - Nes (assay MGI:7768118).
Specimen
S2C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cntnap4; green - Nes (assay MGI:7768118).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex subventricular zone |
neural progenitor cell |
Present |
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S2C Caspr4 inset
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S2C Caspr4
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S2C Caspr4/Nestin inset
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S2C Caspr4/Nestin
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Expression in Nes-expressing neural progenitor cells.
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Specimen
S3A:
postnatal day 0
(more )
Note: Double labeled: red - Cntnap4; green - Tubb3 (assay MGI:7768122).
Specimen
S3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cntnap4; green - Tubb3 (assay MGI:7768122).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: cerebral cortex |
cerebral cortex neuron |
Present |
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S3A Caspr4/Tuj1
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S3A Caspr4
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S3A Caspr4/Tuj1 inset
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S3A Caspr4 inset
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Expression in Tubb3-expressing cortical neurons.
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Specimen
S3E:
postnatal adult
(more )
Note: Double labeled: green - Cntnap4; red - Gfap (assay MGI:7768123).
Specimen
S3E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cntnap4; red - Gfap (assay MGI:7768123).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: corpus callosum |
astrocyte |
Present |
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S3E Caspr4
,
S3E Caspr4/GFAP
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S3E Caspr4/GFAP inset
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S3E Caspr4 inset
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Expression in Gfap-expressing astrocytes.
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Analysis Tools