Notch1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:184017 Chi Z, et al., Botch promotes neurogenesis by antagonizing Notch. Dev Cell. 2012 Apr 17;22(4):707-20
Assay type:	Western blot
MGI Accession ID:	MGI:7767868
Gene symbol:	Notch1
Gene name:	notch 1

Antibody:

Anti-Notch1

Results

Image: 4A

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex	Note
Input	E14.5	TS22: ganglionic eminence	Present	Strong	Not Specified		Not Specified
IP IgG	Control
IP Botch-PoAb	E14.5	TS22: ganglionic eminence	Strong (c)	Present (c)	Not Specified		Not Specified	Sample was immunoprecipitated with the Chac1 polyclonal antibody MGI:7767846.
IP Botch-PoAb + Botch	Control

Notes:
(a) Notch1- uncleaved full length
(b) Notch1- transmembrane and intracellular domain.
(c) Botch/Chac1 immunoprecipitation pulls down more of the uncleaved immature full-length form of Notch1 than the transmembrane and intracellular (TMIC) domain, which is the S1-cleaved mature form of Notch and also the dominant form of Notch in the input.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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