Antibody:	Anti-Cav1.2 (CACNA1C, ACC-003)
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Structure	Cell Type	Level	Pattern	Image	Note
TS19: midgut		Strong	Diffuse	2_CaV1.2 E11.5 , 2_Merge E11.5	Diffuse expression in mesenchyme at E11.5; most intense at outer border and decreased towards interior. Expression in mesenchyme most intense at E11.5; decreased at later stages.
TS19: enteric nervous system	enteric neuron	Absent		2_Merge E11.5 , 2_CaV1.2 E11.5	No expression in enteric neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: midgut		Present	Diffuse	2_Merge E12.5 , 2_CaV1.2 E12.5	Expression is diffuse at E12.5.
TS20: gut muscularis	enteric smooth muscle cell	Absent		2_CaV1.2 E12.5 , 2_Merge E12.5	First detectable circular smooth muscle fibers arise in midgut E12.5. No expression in smooth muscle layer.
TS20: enteric nervous system	enteric neuron	Absent		2_CaV1.2 E12.5 , 2_Merge E12.5	No expression in enteric neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: midgut	enteric smooth muscle cell	Present	Regionally restricted	2_Merge E13.5 , 2_CaV1.2 E13.5	Expression restricted to circular smooth muscle fibers and enteric neurons.
TS21: enteric nervous system	enteric neuron	Present	Regionally restricted	2_CaV1.2 E13.5 , 2_Merge E13.5	Expression more intense in enteric neurons than in surrounding smooth muscle

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midgut	enteric smooth muscle cell	Present	Regionally restricted	2_CaV1.2 E14.5 , 2_Merge E14.5	Expression restricted to circular smooth muscle fibers and enteric neurons.
TS22: enteric nervous system	enteric neuron	Present	Regionally restricted	2_Merge E14.5 , 2_CaV1.2 E14.5	Expression more intense in enteric neurons than in surrounding smooth muscle