Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS19: midgut	Absent		1_E11.5 Proximal midgut	No smooth muscle fibers at E11.5.

Structure	Level	Pattern	Image	Note
TS19: midgut	Absent		1_E11.5 Distal midgut	No smooth muscle fibers at E11.5.

Structure	Level	Pattern	Image	Note
TS19: hindgut	Absent		1_E11.5 Hindgut	No smooth muscle fibers at E11.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E12.5 Proximal midgut	First detectable circular smooth muscle fibers arise in proximal and distal midgut E12.5. Smooth muscle fibers in proximal portions longer and more conspicuous than in distal portions.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E12.5 Distal midgut	First detectable circular smooth muscle fibers arise in proximal and distal midgut at E12.5.

Structure	Level	Pattern	Image	Note
TS20: hindgut	Absent		1_E12.5 Hindgut	No smooth muscle fibers at E12.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E13.5 Proximal midgut	Expression in circular smooth muscle fibers in proximal midgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E13.5 Distal midgut	Expression in circular smooth muscle fibers in distal midgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: hindgut	enteric smooth muscle cell	Present	Regionally restricted	1_E13.5 Hindgut	First detectable circular smooth muscle fibers arise at E13.5 in hindgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E14.5 Proximal midgut	Expression in circular smooth muscle fibers in proximal midgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E14.5 Distal midgut	Expression in circular smooth muscle fibers in distal midgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: hindgut	enteric smooth muscle cell	Present	Regionally restricted	1_E14.5 Hindgut	Expression in circular smooth muscle fibers in hindgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E15.5 Proximal midgut	Expression in circular smooth muscle fibers in proximal midgut. First longitudinal smooth muscle fibers appear in proximal midgut at E15.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: midgut	enteric smooth muscle cell	Present	Regionally restricted	1_E15.5 Distal midgut	Expression in circular smooth muscle fibers in distal midgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: hindgut	enteric smooth muscle cell	Present	Regionally restricted	1_E15.5 Hindgut	Expression in circular smooth muscle fibers in hindgut.