Th Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:108403 Kim SY, et al., The dopamine D2 receptor regulates the development of dopaminergic neurons via extracellular signal-regulated kinase and Nurr1 activation. J Neurosci. 2006 Apr 26;26(17):4567-76
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7748728
Gene symbol:	Th
Gene name:	tyrosine hydroxylase

Antibody:	anti-TH
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Specimen 2A E14 WT: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain tegmentum	midbrain dopaminergic neuron	Present		2A E14 WT left , 2A E14 WT right
TS22: midbrain	midbrain dopaminergic neuron	Present	Regionally restricted	2A E14 WT left , 2A E14 WT right	Expression is in the ventral tegmentum and substantia nigra.

Specimen 2A E14 D2R^-/-: embryonic day 14.5; Drd2^tm1Ebo/Drd2^tm1Ebo (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain tegmentum	midbrain dopaminergic neuron	Weak		2A E14 D2R^-/- right , 2A E14 D2R^-/- left
TS22: midbrain	midbrain dopaminergic neuron	Weak	Regionally restricted	2A E14 D2R^-/- left , 2A E14 D2R^-/- right	The number of expressing cells was significantly reduced in the ventral tegmentum and substantia nigra (70% of WT mice).

Specimen 2A P30 WT SN: postnatal day 30 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: substantia nigra	substantia nigra dopaminergic neuron	Present		2A P30 WT left SN , 2A P30 WT right SN

Specimen 2A P30 D2R^-/- SN: postnatal day 30; Drd2^tm1Ebo/Drd2^tm1Ebo (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: substantia nigra	substantia nigra dopaminergic neuron	Weak		2A P30 D2R^-/- right SN , 2A P30 D2R^-/- left SN	Expression in this mutant was reduced to approximately 62% of wild-type.

Specimen 2A P30 WT VTA: postnatal day 30 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: ventral tegmental area	midbrain dopaminergic neuron	Present		2A P30 WT left VTA , 2A P30 WT right VTA

Specimen 2A P30 D2R^-/- VTA: postnatal day 30; Drd2^tm1Ebo/Drd2^tm1Ebo (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: ventral tegmental area	midbrain dopaminergic neuron	Weak		2A P30 D2R^-/- left VTA , 2A P30 D2R^-/- right VTA	Expression in this mutant was reduced to approximately 54%of wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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