Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85949
Shikama N, et al., Essential function of p300 acetyltransferase activity in heart, lung and small intestine formation. EMBO J. 2003 Oct 1;22(19):5175-85
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7738701
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Probe:
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Bmp4 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4B Bmp4 wt:
embryonic day 16.5
(more  )
Specimen
4B Bmp4 wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung |
Present |
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4B Bmp4 wt
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Specimen
4B Bmp4 +/AS:
embryonic day 16.5; Ep300tm1Reck/Ep300 +
(more )
Specimen
4B Bmp4 +/AS:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ep300tm1Reck/Ep300+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung |
Present |
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4B Bmp4 +/AS
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Expression correctly localized compared to wild type.
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Specimen
5Ba:
embryonic day 16.5
(more )
Specimen
5Ba:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: small intestine mesenchyme |
Strong |
Regionally restricted |
5Ba
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Expression in submucosa. High levels of expression in tip of invading mesenchyme associated with villi formation.
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Specimen
5Bb:
embryonic day 16.5; Ep300tm1Reck/Ep300 +
(more )
Specimen
5Bb:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ep300tm1Reck/Ep300+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5Bc:
embryonic day 16.5
(more )
Specimen
5Bc:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: small intestine mesenchyme |
Strong |
Regionally restricted |
5Bc
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Expression in submucosa. High levels of expression in tip of invading mesenchyme associated with villi formation
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Specimen
5Bd:
embryonic day 16.5; Ep300tm1Reck/Ep300 +
(more )
Specimen
5Bd:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ep300tm1Reck/Ep300+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: small intestine mesenchyme |
Present |
Regionally restricted |
5Bd
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In some mutants, elevated expression marks leading tip of mesenchymal areas beginning to invaginate.
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Analysis Tools