Antibody:	Anti-Spatial-alpha
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Strong		2B , 2A	Expressed in cell bodies.
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present		2C1 , 2C2 , 2A , 2B	More weakly. Expressed in cell bodies/neurons. Not colocalize with the GFAP astrocytes marker.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		2B , 2A	Expressed in cell bodies.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellar glomerulus		Present		2D4 , 2D1 , 2D3	Expressed in cerebellar glomeruli stained with the synaptophysin marker.
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Present		2D1 , 2D3 , 2D4	cytosolic localization

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		2E3 , 2E4 , 2E1	Expressed in the elaborate dendritic tree (Map2+) of Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		2F1 , 2F3 , 2F4	Not expressed in axonal processes (Nefh+).
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Strong		2F4 , 2F3 , 2F1	Not expressed in axonal processes (Nefh+).

Structure	Level	Pattern	Image	Note
TS28: cerebellar glomerulus	Present		2G2 , 2G1 , 2G4	Concentrated in glomerular synaptic structures detected with the synaptophysin marker, although two signals did not overlap.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA3	hippocampal pyramidal neuron	Strong		4B , 4A	Expressed in the cytoplasm and processes.
TS28: hippocampus CA1	hippocampal pyramidal neuron	Strong		4A , 4C	Expressed in the cytoplasm and processes.
TS28: dentate gyrus		Strong		4A

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present		4D1 , 4D3	Colocalizes with the MAP2 somatodendritic marker.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA3	hippocampal pyramidal neuron	Present		4E3 , 4E1	Not colocalize with the GFAP astrocyte marker.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		4F3 , 4F1	Expressed in some synaptic area (Syp+).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus layer	dentate gyrus granule cell	Strong	Regionally restricted	4G2 , 4G3	Strongly detected in the external layer containing neurons under active differentiation while no expression in proliferating neurons of the internal layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus	dentate gyrus granule cell	Present	Widespread	4H2 , 4H3	Continued to express in all granule cells.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present		6C1 , 6C3	Coexpressed with Kif17 in the same vesicles inside the cell body.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		6D3 , 6D1	Coexpressed with Kif17.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer III	cerebral cortex pyramidal neuron	Present		S2D , S2C	Restricted to the cytoplasm of large pyramidal neurons in layer III.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA3	hippocampal pyramidal neuron	Present			Not colocalize with the Galc astrocyte marker.