Probe:	Tbr2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		3U Tbr2 top

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Strong		3U Tbr2 bottom	Expression was enhanced in the subventricular zone of the double mutant.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		5A E15.5 Tbr2 top
TS23: cerebral cortex ventricular layer	Present		5A E15.5 Tbr2 top

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Weak		5A E15.5 Tbr2 bottom	The Eomes-positive subventricular zone was reduced in the double mutant.
TS23: cerebral cortex ventricular layer	Present		5A E15.5 Tbr2 bottom	The thickness of the Eomes-positive ventricular zone was almost unaffected in the double mutant.

Structure	Level	Pattern	Image	Note
TS24: pallium	Present	Regionally restricted	S8A	Expression was detected in the pallium ventricular zone and subventricular zone. The ventral limit of expression was observed as the pallial/subpallial boundary.

Structure	Level	Pattern	Image	Note
TS24: pallium	Present	Regionally restricted	S8B	Expression was detected in the pallium ventricular zone and subventricular zone. The ventral limit of expression indicated that the pallial/subpallial boundary shifted dorsally in the double mutant.