Tbr1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:170671 Shibata M, et al., MicroRNA-9 regulates neurogenesis in mouse telencephalon by targeting multiple transcription factors. J Neurosci. 2011 Mar 2;31(9):3407-22
Assay type:	RNA in situ
MGI Accession ID:	MGI:7736349
Gene symbol:	Tbr1
Gene name:	T-box brain transcription factor 1

Probe:	Tbr1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3S ©

Specimen 3S: embryonic day 12.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cortical preplate	cerebral cortex neuron	Present	Regionally restricted	3S	Early differentiated neurons were labeled.

3T ©

Specimen 3T: embryonic day 12.5; Mir9-2^tm1Sia/Mir9-2^tm1Sia, Mir9-3^tm1Sia/Mir9-3^tm1Sia (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cortical preplate	cerebral cortex neuron	Weak	Regionally restricted	3T	Early differentiated neurons were significantly reduced in the preplate.

8A top Tbr1 ©

Specimen 8A miR-9-2 +/+; miR9-3 +/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8A top Tbr1	Expression was detected in layer VI.

Specimen 8A miR-9-2 -/-; miR9-3 -/-: embryonic day 18.5; Mir9-2^tm1Sia/Mir9-2^tm1Sia, Mir9-3^tm1Sia/Mir9-3^tm1Sia (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8A bottom Tbr1	Tbr1-positive layer VI was almost normally generated.

8C Tbr1 left ©

Specimen 8C miR-9-2 +/+; miR9-3 +/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8C Tbr1 left	Expression was detected in layer VI.

Specimen 8C miR-9-2 -/-; miR9-3 -/-: embryonic day 18.5; Mir9-2^tm1Sia/Mir9-2^tm1Sia, Mir9-3^tm1Sia/Mir9-3^tm1Sia (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8C Tbr1 right	Tbr1-positive layer VI was almost normally generated.

S4B Tbr1 top ©

Specimen S4B miR-9-2 +/+; miR9-3 +/+: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	S4B Tbr1 top	Expression was detected in layer VI.

Specimen S4B miR-9-2 -/-; miR9-3 +/+: embryonic day 16.5; Mir9-2^tm1Sia/Mir9-2^tm1Sia (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	S4B Tbr1 bottom	Single homozygous mutants didn't exhibit any apparent defects in neurogenesis.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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