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Gene Expression Data
Assay Details
Assay
Reference: J:354187 Hayashi Y, et al., Control of epigenomic landscape and development of fetal male germ cells through L-serine metabolism. iScience. 2024 Sep 20;27(9):110702
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7736249
Gene symbol: Phgdh
Gene name: 3-phosphoglycerate dehydrogenase
Antibody: anti-3PGDH (MSFR100010)
Detection system: Secondary antibody
Results
Specimen 1B E12.5 male: embryonic day 12.5 (more )
Note: Double labeled: green - Phgdh; red - Ddx4 (assay MGI:7736248).
Structure Level Pattern Image Note
TS20: germ cell of testis Weak 1B E12.5 male PHGDH , 1B E12.5 male (merged) Expression significantly reduced in germ cells compared to somatic cells.
TS20: testis Present Regionally restricted 1B E12.5 male (merged) , 1B E12.5 male PHGDH
Specimen 1B E12.5 female: embryonic day 12.5 (more )
Note: Double labeled: green - Phgdh; red - Ddx4 (assay MGI:7736248).
Structure Level Pattern Image Note
TS20: germ cell of ovary Weak 1B E12.5 female (merged) , 1B E12.5 female PHGDH Expression significantly reduced in germ cells compared to somatic cells.
TS20: ovary Present Regionally restricted 1B E12.5 female (merged) , 1B E12.5 female PHGDH
Specimen 1B E16.5 male: embryonic day 16.5 (more )
Note: Double labeled: green - Phgdh; red - Ddx4 (assay MGI:7736248).
Structure Level Pattern Image Note
TS24: germ cell of testis Strong 1B E16.5 male PGHDH , 1B E16.5 male (merged) Marked upregulation in expression in germ cells compared to somatic cells.
TS24: testis Present Regionally restricted 1B E16.5 male (merged) , 1B E16.5 male PGHDH
Specimen 1B E16.5 female: embryonic day 16.5 (more )
Note: Double labeled: green - Phgdh; red - Ddx4 (assay MGI:7736248).
Structure Level Pattern Image Note
TS24: germ cell of ovary Weak 1B E16.5 female PGHDH , 1B E16.5 female (merged)
TS24: ovary Present Regionally restricted 1B E16.5 female PGHDH , 1B E16.5 female (merged)
Specimen S2A P0 testis: postnatal day 0 (more )
Note: Double labeled: green - Phgdh; red - Ddx4 (assay MGI:7736248).
Structure Level Pattern Image Note
TS27: germ cell of testis Strong S2A PHGDH , S2A (merged) Relatively elevated expression in germ cells compared to somatic cells persisted postnatally.
TS27: testis Present Regionally restricted S2A (merged) , S2A PHGDH
Specimen S2B P7 testis: postnatal day 7 (more )
Note: Double labeled: green - Phgdh; red - Ddx4 (assay MGI:7736248).
Structure Level Pattern Image Note
TS28: germ cell of testis Strong S2B PHGDH , S2B (merged) Relatively elevated expression in germ cells compared to somatic cells persisted postnatally.
TS28: testis Present Regionally restricted S2B PHGDH , S2B (merged)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory