Gene Expression Data
Assay Details
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Assay
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Reference:
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J:196918
Iskratsch T, et al., Two distinct phosphorylation events govern the function of muscle FHOD3. Cell Mol Life Sci. 2013 Mar;70(5):893-908
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7734908
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Gene symbol:
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Fhod3
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Gene name:
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formin homology 2 domain containing 3
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Results
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Specimen
2B E9.5:
embryonic day 9.5
(more  )
Note: Double labeled: green - Fhod3; red - Tnnt1 (assay MGI:7734909).
Specimen
2B E9.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fhod3; red - Tnnt1 (assay MGI:7734909).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: cardiac muscle tissue |
cardiac muscle cell |
Present |
Regionally restricted |
2B E9.5 Merge (upper)
,
2B E9.5 anti-FHOD3 (lower)
,
2B E9.5 Merge (lower)
,
2B E9.5 anti-FHOD3 (upper)
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Expression in cardiomyocytes as early as E9.5 in a diffuse pattern with more intense expression along microfibrils
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Specimen
2B E13.5:
embryonic day 13.5
(more )
Note: Double labeled: green - Fhod3; red - Tnnt1 (assay MGI:7734909).
Specimen
2B E13.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fhod3; red - Tnnt1 (assay MGI:7734909).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cardiac muscle tissue |
Present |
Regionally restricted |
2B E13.5 Merge (lower)
,
2B E13.5 Merge (upper)
,
2B E13.5 anti-FHOD3 (lower)
,
2B E13.5 anti-FHOD3 (upper)
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Expression becomes restricted and mostly found around Z-discs.
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Specimen
2B Adult:
postnatal adult
(more )
Note: Double labeled: green - Fhod3; red - Myom1 (assay MGI:7734913).
Specimen
2B Adult:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fhod3; red - Myom1 (assay MGI:7734913).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cardiac muscle tissue |
Present |
Regionally restricted |
2B Adult anti-FHOD3 (lower)
,
2B Adult anti-Myomesin (lower)
,
2B Adult anti-Myomesin (upper)
,
2B Adult anti-FHOD3 (upper)
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Expression exclusively found at Z-discs, with a clear alternating pattern with myomesin.
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Specimen
4A wt:
embryonic day 9.5
(more )
Note: Double labeled: green - Fhod3; red - myosin.
Specimen
4A wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fhod3; red - myosin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: cardiac muscle tissue |
Present |
Regionally restricted |
4A Merge (upper)
,
4A anti-FHOD (upper)
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4A Merge (lower)
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4A anti-FHOD (lower)
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Expression clearly associated with myofibrils.
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Specimen
4B CK2-/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Csnk2a1tm1Dcs/Csnk2a1tm1Dcs
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fhod3; red - myosin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: cardiac muscle tissue |
Present |
Regionally restricted |
4B Merge (lower)
,
4B Merge (upper)
,
4B anti-FHOD (lower)
,
4B anti-FHOD (upper)
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Pattern is more diffuse compared to wild type. Clear microfibrillar association not observed. Increased number of speckles is observed, similar to that obsesrved for non-muscle form.
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Analysis Tools