Antibody:	anti-beta3-tubulin
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Structure	Cell Type	Level	Pattern	Image	Note
TS28: modiolus	spiral ganglion neuron	Present	Regionally restricted	1	Expressed in the basal turns, middle turns and apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	2a	Positive Rosenthal's canals existed at the basal turn and the middle turn.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	2b	Positive Rosenthal's canals existed at all basal to apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	2c	Positive Rosenthal's canals existed at all basal to apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: osseus spiral lamina	spiral ganglion neuron	Present		2d	Positive Rosenthal's canals existed at all basal to apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	2e	Positive Rosenthal's canals existed at the basal turn, but not at the middle and apical turns in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	2f	Positive Rosenthal's canals existed at all basal to apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	2g	Positive Rosenthal's canals existed at all basal to apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: osseus spiral lamina	spiral ganglion neuron	Present		2h	Positive Rosenthal's canals existed at all basal to apical turns.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	3a	Positive cells were shown in the basal turn.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	3b	Positive cells were shown in the basal turn.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	3c	Positive cells were shown in the basal turn.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: osseus spiral lamina	spiral ganglion neuron	Present	Regionally restricted	3d	Positive cells were shown in the basal turn.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	3e	Fewer positive cells were shown in the basal turn, compared with the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	3f	Fewer positive cells were shown in the basal turn, compared with the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: bony labyrinth	spiral ganglion neuron	Present	Regionally restricted	3g	Fewer positive cells were shown in the basal turn, compared with the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: osseus spiral lamina	spiral ganglion neuron	Present	Regionally restricted	3h	Fewer positive cells were shown in the basal turn, compared with the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: bony labyrinth	spiral ganglion neuron	Present			The total number of positive cells were 140+/-60.7.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: bony labyrinth	spiral ganglion neuron	Present			The total number of positive cells were 78+/-4.3, significantly fewer compared with the wild-type. Comparison of the number at at each turn revealed significantly fewer positive cells in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: bony labyrinth	spiral ganglion neuron	Present			The total number of positive cells were 282+/-45.9.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: bony labyrinth	spiral ganglion neuron	Present			The total number of positive cells were 201+/-19.8, significantly fewer compared with the wild-type. Comparison of the number at at each turn revealed significantly fewer positive cells in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: bony labyrinth	spiral ganglion neuron	Present			The total number of positive cells were 288+/-22.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: bony labyrinth	spiral ganglion neuron	Present			The total number of positive cells were 232+/-16.2, significantly fewer compared with the wild-type. Comparison of the number at at each turn revealed significantly fewer positive cells in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: osseus spiral lamina	spiral ganglion neuron	Present			The total number of positive cells were 300+/-13.6.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: osseus spiral lamina	spiral ganglion neuron	Present			The total number of positive cells were 239+/-21.7, significantly fewer compared with the wild-type. Comparison of the number at at each turn revealed significantly fewer positive cells in the mutant.