Rb1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:55690 Geng Y, et al., Rescue of cyclin D1 deficiency by knockin cyclin E. Cell. 1999 Jun 11;97(6):767-77
Assay type:	Western blot
MGI Accession ID:	MGI:7734670
Gene symbol:	Rb1
Gene name:	RB transcriptional corepressor 1

Antibody:	Anti-Retinoblastoma G3-245 (#554136)
Detection system:	horseradish peroxidase

Results

Image: 7C pRB

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex	Note
retinas WT	P adult	TS28: retina	Strong (a)	involves: 129/Sv * C57BL/6		Not Specified
retinas KI	P adult	TS28: retina	Weak (b)	involves: 129/Sv * C57BL/6	Ccnd1^{tm1(CCNE)Pisc}/Ccnd1^{tm1(CCNE)Pisc}	Not Specified
retinas KO	P adult	TS28: retina	Weak (c)	involves: 129S2/SvPas * C57BL/6	Ccnd1^tm1Wbg/Ccnd1^tm1Wbg	Not Specified
retinas KI	P adult	TS28: retina	Weak (b)	involves: 129/Sv * C57BL/6	Ccnd1^{tm1(CCNE)Pisc}/Ccnd1^{tm1(CCNE)Pisc}	Not Specified
mammary gland WT	P adult	TS28: mammary gland	Strong	involves: 129/Sv * C57BL/6		Female	Pregnant at day 16.5 of gestation.
mammary gland KI	P adult	TS28: mammary gland TS28: retina	Weak (d)	involves: 129/Sv * C57BL/6	Ccnd1^{tm1(CCNE)Pisc}/Ccnd1^{tm1(CCNE)Pisc}	Female	Pregnant at day 16.5 of gestation.

Notes:
(a) High level of phosphorylated pRB.
(b) Phosphorylation levels approximately 35% compared to wild type.
(c) Proportion of hyperphosphorylated pRB reduced to approximately 23%.
(d) Phosphorylation levels decreased compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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