Antibody:	Anti-Calcitonin
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS25: thyroid gland	parafollicular cell	Present		1N , 1S

Structure	Cell Type	Level	Pattern	Image	Note
TS24: thyroid gland	parafollicular cell	Present	Scattered	3D	Expressed in in C-cells scattered throughout the gland in a parafollicular position.

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Present	Clusters	3I	Positive cells were clustered in a specific region. The positive surface area relative to the Nkx2-1-positive surface area was smaller than in wild type mice (see Fig 4).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: thyroid gland	parafollicular cell	Present	Scattered	3N

Structure	Level	Pattern	Image	Note
TS23: thyroid gland	Present	Single cells	3S	Few positive cells were seen in the mutant, localized at the cranial part.