Gene Expression Data
Assay Details
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Assay
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Reference:
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J:77237
Verhage M, et al., Synaptic assembly of the brain in the absence of neurotransmitter secretion. Science. 2000 Feb 4;287(5454):864-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7718353
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Gene symbol:
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Adgre1
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Gene name:
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adhesion G protein-coupled receptor E1
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Antibody:
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anti-F4/80
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Fixation was with 71.4% saturated picric acid, 23.8% formalin, and 4.8% acetic acid.
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Results
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Specimen
5E:
embryonic day 18.0
(more  )
Note: Data from wild-type and heterozygous embryos were pooled as the control group after pilot experiments revealed no differences between these groups.
Specimen
5E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Data from wild-type and heterozygous embryos were pooled as the control group after pilot experiments revealed no differences between these groups.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: hippocampus |
macrophage |
Present |
Regionally restricted |
5E
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Expression in activated macrophages, largely restricted to ventricles.
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| TS26: thalamus |
macrophage |
Present |
Regionally restricted |
5E
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Expression in activated macrophages, largely restricted to ventricles.
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Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hippocampus |
Present |
Widespread |
5F
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Expression throughout tissue.
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| TS26: thalamus |
Present |
Widespread |
5F
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Expression throughout tissue.
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Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
macrophage |
Present |
Regionally restricted |
5H
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Expression colocalized with apoptotic cell bodies, probably engulfed by macrophages.
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Analysis Tools
