Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:315095
Bando Y, et al., Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice. J Mol Histol. 2014 Oct;45(5):507-18
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7716430
|
|
Gene symbol:
|
Fabp5
|
|
Gene name:
|
fatty acid binding protein 5, epidermal
|
|
Antibody:
|
Anti-EFABP
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
1f:
postnatal week 4
(more  )
Note: Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
Specimen
1f:
(close  )
|
Genetic Background:
|
DDY
|
|
Age:
|
postnatal week 4
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate ossification zone |
perivascular cell |
Present |
Regionally restricted |
1f
|
Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with cathepsin B-positive (Ctsb) cells, which identified the cells as septoclasts/perivascular cells.
|
| TS28: tibia |
perivascular cell |
Present |
Regionally restricted |
1f
|
Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with cathepsin B-positive (Ctsb) cells, which identified the cells as septoclasts/perivascular cells.
|
Specimen
1g:
postnatal week 4
(more )
Note: Double labeled: green - Fabp5; red - DAB lectin.
Specimen
1g:
(close )
|
Genetic Background:
|
DDY
|
|
Age:
|
postnatal week 4
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fabp5; red - DAB lectin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate ossification zone |
Present |
Regionally restricted |
1g
|
Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with DBA lectin-positive cells, which identified the cells as septoclasts/perivascular cells.
|
| TS28: tibia |
Present |
Regionally restricted |
1g
|
Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with DBA lectin-positive cells, which identified the cells as septoclasts/perivascular cells.
|
Specimen
2b:
postnatal week 4
(more )
Note: Double labeled: green - Fabp5; red - BSA lectin.
Specimen
2b:
(close )
|
Genetic Background:
|
DDY
|
|
Age:
|
postnatal week 4
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fabp5; red - BSA lectin.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate ossification zone |
endothelial cell |
Absent |
|
2b
|
Expression did not overlap with BSA lectin stained endothelial cells of the proximal tibia.
|
Specimen
6b:
embryonic day 16.5
(more )
Note: Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
Specimen
6b:
(close )
|
Genetic Background:
|
DDY
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Applicable
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: epiphyseal plate |
Present |
Regionally restricted |
6b
|
Double immunostaining for Fabp5 and cathepsin B (Ctsb), cells showing co-localization of both proteins were observed in the growth plate.
|
|
Analysis Tools