Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:43434
George EL, et al., Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. Blood. 1997 Oct 15;90(8):3073-81
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7715145
|
|
Gene symbol:
|
Pecam1
|
|
Gene name:
|
platelet/endothelial cell adhesion molecule 1
|
|
Probe:
|
PECAM-1 probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4B +/+:
embryonic day 8.5
(more  )
Specimen
4B +/+:
(close  )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4B -/- (left):
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Fn1tm1Hyn/Fn1tm1Hyn
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: vascular system |
Present |
Scattered |
4B -/- (left)
|
Fewer expressing cells, more widely dispersed compared to wild type.
|
Specimen
4B -/- (right):
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Fn1tm1Hyn/Fn1tm1Hyn
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: vascular system |
Present |
Scattered |
4B -/- (right)
|
Fewer expressing cells, more widely dispersed compared to wild type.
|
Specimen
4C +/+:
embryonic day 8.5
(more )
Specimen
4C +/+:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4C -/-:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Fn1tm1Hyn/Fn1tm1Hyn
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: dorsal aorta |
Present |
Regionally restricted |
4C -/-
|
In embryos with less severe phenotype, normal number and pattern of expressing endothelial cells.
|
Specimen
4D +/+:
embryonic day 8.5
(more )
Specimen
4D +/+:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4D -/-:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Fn1tm1Hyn/Fn1tm1Hyn
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: dorsal aorta |
Present |
Regionally restricted |
4D -/-
|
In embryos with less severe phenotype, normal number and pattern of expressing endothelial cells.
|
|
Analysis Tools