Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:349792 Ansari SS, et al., Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length. J Cell Biol. 2024 Sep 2;223(9)
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7714612
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:	anti PAX6 (DSHB)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

6C +/+ merge ©

6C +/+ PAX6 ©

Specimen 6C Ep4+/+: embryonic day 9.5 (more

)
Note: 25-29 somite stage embryo. Triple labeled: green - Pax6; magenta - Olig2 (assay MGI:7714611); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		6C +/+ PAX6 , 6C +/+ merge

6C +/- merge ©

6C +/- PAX6 ©

Specimen 6C Ep4+/-: embryonic day 9.5; Ptger4^tm1Sna/Ptger4⁺ (more

)
Note: 25-29 somite stage embryo. Triple labeled: green - Pax6; magenta - Olig2 (assay MGI:7714611); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		6C +/- merge , 6C +/- PAX6

6C -/- merge ©

6C -/- PAX6 ©

Specimen 6C Ep4-/-: embryonic day 9.5; Ptger4^tm1Sna/Ptger4^tm1Sna (more

)
Note: 25-29 somite stage embryo. Triple labeled: green - Pax6; magenta - Olig2 (assay MGI:7714611); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		6C -/- merge , 6C -/- PAX6	The expressing domain is comparable in size to that seen in wild type, but it has shifted ventrally coincident with the reduction of the Olig2-positive progenitor population.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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