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Gene Expression Data
Assay Details
Assay
Reference: J:293992 Mancia Leon WR, et al., Clustered gamma-protocadherins regulate cortical interneuron programmed cell death. Elife. 2020 Jul 7;9:e55374
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7713225
Gene symbol: Casp3
Gene name: caspase 3
Results
Specimen 5A Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 7; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: blue/white - Casp3; red - tdTomato.
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex inhibitory interneuron Present Regionally restricted 5A CC3 + , 5A Merged Similarly to their wild type littermates, Nkx2.1<cre>;Ai14;Pcdh<fcon3/fcon3> homozygous mice displayed a wave of programmed cell death peaking at P7. Casp3+;tdTomato+ were observed in the mutant cortex. However, the mutant mice had significantly higher numbers of tdTomato+/Casp3+ cells compared to controls.
TS28: cerebral cortex Present Regionally restricted 5A CC3 + , 5A Merged Expression was also observed in Casp3+ cells that were tdTomato negative, un-recombined cells that would notably include pyramidal cells, CGE-derived cortex inhibitory neurons, and glial cells. No significant difference in the number of Casp3+/tdTomato- cells was observed between genotypes.

Specimen not shown P7; Nkx2.1Cre;Ai14;WT: postnatal day 7; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex inhibitory interneuron Present Regionally restricted A wave of programmed cell death starts at ~P0, peaks at P7, and ends at ~P15.

Specimen not shown P0; Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 0; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex inhibitory interneuron Present Regionally restricted Similarly to their wild type littermates, Nkx2.1<cre>;Ai14;Pcdh<fcon3/fcon3> homozygous mice displayed a wave of programmed cell death. Casp3+;tdTomato+ cells were observed in the mutant cortex.
TS27: cerebral cortex Present Regionally restricted Expression was also observed in Casp3+ cells that were tdTomato negative, un-recombined cells that would notably include pyramidal cells, CGE-derived cortex inhibitory neurons, and glial cells. A small, but significant, difference in the number of Casp3+/tdTomato- cells was observed between genotypes at P0.

Specimen not shown P0; Nkx2.1Cre;Ai14;WT: postnatal day 0; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex inhibitory interneuron Present Regionally restricted A wave of programmed cell death starts at ~P0, peaks at P7, and ends at ~P15.

Specimen not shown P3; Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 3; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex inhibitory interneuron Present Regionally restricted Similarly to their wild type littermates, Nkx2.1<cre>;Ai14;Pcdh<fcon3/fcon3> homozygous mice displayed a wave of programmed cell death. Casp3+;tdTomato+ cells were observed in the mutant cortex. However, the mutant mice had significantly higher numbers of tdTomato+/Casp3+ cells compared to controls.
TS27: cerebral cortex Present Regionally restricted Expression was also observed in Casp3+ cells that were tdTomato negative, un-recombined cells that would notably include pyramidal cells, CGE-derived cortex inhibitory neurons, and glial cells. No significant difference in the number of Casp3+/tdTomato- cells was observed between genotypes.

Specimen not shown P3; Nkx2.1Cre;Ai14;WT: postnatal day 3; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex inhibitory interneuron Present Regionally restricted A wave of programmed cell death starts at ~P0, peaks at P7, and ends at ~P15.

Specimen not shown P10; Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 10; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex inhibitory interneuron Present Regionally restricted Similarly to their wild type littermates, Nkx2.1<cre>;Ai14;Pcdh<fcon3/fcon3> homozygous mice displayed a wave of programmed cell death. Casp3+;tdTomato+ cells were observed in the mutant cortex.
TS28: cerebral cortex Present Regionally restricted Expression was also observed in Casp3+ cells that were tdTomato negative, un-recombined cells that would notably include pyramidal cells, CGE-derived cortex inhibitory neurons, and glial cells. No significant difference in the number of Casp3+/tdTomato- cells was observed between genotypes.

Specimen not shown P10; Nkx2.1Cre;Ai14;WT: postnatal day 10; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex inhibitory interneuron Present Regionally restricted A wave of programmed cell death starts at ~P0, peaks at P7, and ends at ~P15.

Specimen not shown P15; Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 15; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex inhibitory interneuron Ambiguous

Specimen not shown P15; Nkx2.1Cre;Ai14;WT: postnatal day 15; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex inhibitory interneuron Ambiguous A wave of programmed cell death starts at ~P0, peaks at P7, and ends at ~P15.


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last database update
01/28/2026
MGI 6.24
The Jackson Laboratory