Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74066
Fauchey V, et al., Dopamine control of striatal gene expression during development: relevance to knockout mice for the dopamine transporter. Eur J Neurosci. 2000 Sep;12(9):3415-25
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|
Assay type:
|
RNA in situ
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|
MGI Accession ID:
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MGI:7713123
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|
Gene symbol:
|
Drd2
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Gene name:
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dopamine receptor D2
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Probe:
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D2 probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1B D2 DAT+/+:
embryonic day 13.0
(more  )
Specimen
1B D2 DAT+/+:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
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|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: corpus striatum |
Present |
|
1B D2 DAT+/+
|
Expression in striatal anlage.
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Specimen
1B D2 DAT-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: corpus striatum |
Present |
|
1B D2 DAT-/-
|
Expression in striatal anlage.
|
Specimen
D2 G14 not shown:
embryonic day 14.0
(more )
Specimen
D2 G14 not shown:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Present |
Non-Uniform |
|
Expression heterogeneously distributed throughout striatum.
|
Specimen
D2 G16 not shown:
embryonic day 16.0
(more )
Specimen
D2 G16 not shown:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: corpus striatum |
Present |
Non-Uniform |
|
Expression heterogeneously distributed throughout striatum.
|
Specimen
2B D2 DAT+/+:
embryonic day 17.0
(more )
Specimen
2B D2 DAT+/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Present |
Non-Uniform |
2B D2 DAT+/+
|
Expression heterogeneously distributed throughout striatum.
|
Specimen
2B D2 DAT-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Weak |
|
2B D2 DAT-/-
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Expression decreased compared to wild type.
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Specimen
3B D2 DAT+/+:
postnatal day 2
(more )
Specimen
3B D2 DAT+/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
Graded |
3B D2 DAT+/+
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Expression heterogeneously distributed throughout striatum, with increasing mediolateral gradient of expression.
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Specimen
3B D2 DAT-/-:
(close )
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Genetic Background:
|
involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
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|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Weak |
|
3B D2 DAT-/-
|
Expression decreased compared to wild type.
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Specimen
D2 whole striatum G13 +/+:
embryonic day 13.0
(more )
Specimen
D2 whole striatum G13 +/+:
(close )
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Genetic Background:
|
involves: 129P2/OlaHsd
|
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Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
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Embedding:
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Cryosection
|
Specimen
D2 whole striatum G13 -/-:
(close )
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Genetic Background:
|
involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 whole striatum G14 +/+:
embryonic day 14.0
(more )
Specimen
D2 whole striatum G14 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 whole striatum G14 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Weak |
|
|
Expression decreased by 36% compared to wild type.
|
Specimen
D2 whole striatum G16 +/+:
embryonic day 16.0
(more )
Specimen
D2 whole striatum G16 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 whole striatum G16 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: corpus striatum |
Weak |
|
|
Expression decreased by 61% compared to wild type.
|
Specimen
D2 whole striatum G17 +/+:
embryonic day 17.0
(more )
Specimen
D2 whole striatum G17 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 whole striatum G17 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Weak |
|
|
Expression decreased by 42% compared to wild type.
|
Specimen
D2 caudate-putamen P0 +/+:
postnatal day 0
(more )
Specimen
D2 caudate-putamen P0 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 0
|
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Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 caudate-putamen P0 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: caudate-putamen |
Weak |
|
|
Expression decreased by 46% compared to wild type.
|
Specimen
D2 caudate-putamen P2 +/+:
postnatal day 2
(more )
Specimen
D2 caudate-putamen P2 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 caudate-putamen P2 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 caudate-putamen P7 +/+:
postnatal day 7
(more )
Specimen
D2 caudate-putamen P7 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 caudate-putamen P7 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 caudate-putamen Adult +/+:
postnatal adult
(more )
Specimen
D2 caudate-putamen Adult +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 caudate-putamen Adult -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: caudate-putamen |
Weak |
|
|
Expression decreased by 36% compared to wild type.
|
Specimen
D2 nucleus accumbens P0 +/+:
postnatal day 0
(more )
Specimen
D2 nucleus accumbens P0 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens P0 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens P2 +/+:
postnatal day 2
(more )
Specimen
D2 nucleus accumbens P2 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens P2 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens P7 +/+:
postnatal day 7
(more )
Specimen
D2 nucleus accumbens P7 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens P7 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens Adult +/+:
postnatal adult
(more )
Specimen
D2 nucleus accumbens Adult +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
D2 nucleus accumbens Adult -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools