Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:106811
Johnson EB, et al., Abnormal development of the apical ectodermal ridge and polysyndactyly in Megf7-deficient mice. Hum Mol Genet. 2005 Nov 15;14(22):3523-38
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7712821
|
|
Gene symbol:
|
Wnt7a
|
|
Gene name:
|
wingless-type MMTV integration site family, member 7A
|
|
Probe:
|
Wnt7a probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
S3 Forelimb WT:
embryonic day 10.5
(more  )
Note: Wild type was either +/+ or heterozygous.
Specimen
S3 Forelimb WT:
(close  )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Wild type was either +/+ or heterozygous.
|
Specimen
S3 Hindlimb WT:
embryonic day 10.5
(more )
Note: Wild type was either +/+ or heterozygous.
Specimen
S3 Hindlimb WT:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Wild type was either +/+ or heterozygous.
|
Specimen
S3 Forelimb KO:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Mutant Allele(s):
|
Lrp4tm1Her/Lrp4tm1Her
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
S3_F KO
|
In this mutant expression in the dorsal ectoderm is displaced proximally from the distal zone of the bud compared to wild-type.
|
Specimen
S3 Hindlimb KO:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Mutant Allele(s):
|
Lrp4tm1Her/Lrp4tm1Her
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud ectoderm |
Present |
Regionally restricted |
S3_H KO
|
In this mutant expression in the dorsal ectoderm is displaced proximally from the distal zone of the bud compared to wild-type.
|
|
Analysis Tools