Antibody:	anti-Keratin 5
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	basal cell of epidermis	Strong		3b	Strong expression in basal cells.
TS27: epidermis suprabasal layer	suprabasal keratinocyte	Weak	Regionally restricted	3b	Expression downregulated within cells of suprabasal levels.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	basal cell of epidermis	Strong		3i	Expression not altered compared to wild type.
TS27: epidermis suprabasal layer	suprabasal keratinocyte	Weak		3i	Expression not altered compared to wild type.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present		5a
TS15: somite	Present	Regionally restricted	5a	Distinct expression in ectoderm lining borders of somites.

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process	Strong		5b	Expression more pronounced in frontonasal mass.
TS17: head surface ectoderm	Weak		5b	Expression less prominent in surface ectoderm of cranial region.

Structure	Level	Pattern	Image	Note
TS20: cranium	Ambiguous		5c	Almost no expression in cranial vault.
TS20: fronto-nasal process epithelium	Strong		5c	Strong expression in ectoderm of frontonasal mass.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present		5d
TS15: somite	Present	Regionally restricted	5d	Distinct expression in ectoderm lining borders of somites.

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process	Weak		5e	Reduced expression in frontonasal region.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Uniform	5f	Expression evenly distributed in surface ectoderm.