Gene Expression Data
Assay Details
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Assay
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Reference:
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J:278827
Murcia-Belmonte V, et al., A Retino-retinal Projection Guided by Unc5c Emerged in Species with Retinal Waves. Curr Biol. 2019 Apr 1;29(7):1149-1160.e4
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7709423
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Gene symbol:
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Ntn1
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Gene name:
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netrin 1
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Probe:
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Netrin1 probe
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Probe preparation:
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Antisense labelled with Alexa Fluor 647 RNA
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Visualized with:
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Fluorescence
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Results
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Specimen
2A:
embryonic day 12.5
(more  )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: optic chiasma |
Strong |
Regionally restricted |
2A
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Expressed ventrally in the chiasma, and detected strongly at the level of the future optic tracts.
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Specimen
2A':
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2A':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: optic chiasma |
Strong |
Regionally restricted |
2A'
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Expressed ventrally in the chiasma, and detected strongly at the level of the future optic tracts.
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Specimen
2A":
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2A":
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: optic chiasma |
Strong |
Regionally restricted |
2A"
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Expressed ventrally in the chiasma, and detected strongly at the level of the future optic tracts.
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Specimen
2B:
embryonic day 14.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
2B
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Continued to be expressed at the chiasma region and surrounded retinal ganglion cells axons at their most ventral aspect.
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Specimen
2B':
embryonic day 14.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2B':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
2B'
|
Continued to be expressed at the chiasma region and surrounded retinal ganglion cells axons at their most ventral aspect.
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Specimen
2B":
embryonic day 14.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2B":
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
2B"
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Continued to be expressed at the chiasma region and surrounded retinal ganglion cells axons at their most ventral aspect.
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Specimen
2C:
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: optic chiasma |
Strong |
Regionally restricted |
2C
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Expressed ventrally in the chiasma, and detected strongly at the level of the future optic tracts.
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Specimen
2C':
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2C':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: optic chiasma |
Strong |
Regionally restricted |
2C'
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Expressed ventrally in the chiasma, and detected strongly at the level of the future optic tracts.
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Specimen
2C":
embryonic day 12.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2C":
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: optic chiasma |
Strong |
Regionally restricted |
2C"
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Expressed ventrally in the chiasma, and detected strongly at the level of the future optic tracts.
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Specimen
2D:
embryonic day 14.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
2D
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Continued to be expressed at the chiasma region and surrounded retinal ganglion cells axons at their most ventral aspect.
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Specimen
2D':
embryonic day 14.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2D':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
2D'
|
Continued to be expressed at the chiasma region and surrounded retinal ganglion cells axons at their most ventral aspect.
|
Specimen
2D":
embryonic day 14.5
(more )
Note: Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
Specimen
2D":
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Ntn1; green - Tubb3 (assay MGI:7709424).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
2D"
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Continued to be expressed at the chiasma region and surrounded retinal ganglion cells axons at their most ventral aspect.
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Analysis Tools