Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:317277
Eildermann K, et al., Developmental expression of the pluripotency factor sal-like protein 4 in the monkey, human and mouse testis: restriction to premeiotic germ cells. Cells Tissues Organs. 2012;196(3):206-20
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7708139
|
Gene symbol:
|
Sall4
|
Gene name:
|
spalt like transcription factor 4
|
Antibody:
|
anti-SALL4 (6E3) (ab57577)
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
|
Specimen
4d:
embryonic day 12.5
(more )
Specimen
4d:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: germ cell of testis |
Present |
Regionally restricted |
4d
|
Homogeneous expression within nucleus in some cells; peripheral nuclear expression in other cells.
|
Specimen
4d inset:
embryonic day 12.5
(more )
Specimen
4d inset:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
4e:
postnatal day 3
(more )
Specimen
4e:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 3
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: spermatogonium |
Strong |
Regionally restricted |
4e
|
Strong expression in immature spermatogonia. Homogenous expression within nucleus.
|
Specimen
4f:
postnatal day 9
(more )
Specimen
4f:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 9
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
Moderate |
|
4f
|
Moderate or strong expression predominantly in spermatogonia.
|
Specimen
4g:
postnatal day 27
(more )
Specimen
4g:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 27
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
Strong |
|
4g
|
Strong expression very predominantly in spermatogonia during all stages of spermatogenic cycle.
|
Specimen
4h:
postnatal adult
(more )
Specimen
4h:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
Strong |
|
4h
|
Strong expression very predominantly in spermatogonia during all stages of spermatogenic cycle.
|
TS28: spermatid |
Trace |
|
4h
|
Sometimes very faint expression in meiotic spermatocytes and post meiotic spermatids.
|
TS28: spermatocyte |
Trace |
|
4h
|
Sometimes very faint expression in meiotic spermatocytes and post meiotic spermatids.
|
|