Antibody:	anti-Zic2
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS23: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted	1d , 1b	Expression was detected in ipsilateral retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label was seen with Isl1, which labels all postmitotic RGC's. Few of the RGC's were co-labeled with EdU when injected at E11.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted	1h , 1f	Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. Co-label was seen with Isl1, which labels all postmitotic RGC's. Few of the RGC's were co-labeled with EdU when injected at E12.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted	1j , 1l	Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. Co-label was seen with Isl1, which labels all postmitotic RGC's. The number of RGC's co-labeled with EdU was higher when injected at E13 than when injected at previous ages.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted	1p , 1n	Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. Co-label was seen with Isl1, which labels all postmitotic RGC's. The number of RGC's co-labeled with EdU was highest when injected at E14 than when injected at previous ages.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted		Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU when injected at E14 was similar between samples taken at E15.5 and E16.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted		Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU when injected at E15 was similar to when injected at E14.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted		Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. Some RGC's were co-labeled with EdU when injected at E13.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted		Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU was similar when injected at E14 than when injected at E13.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted		Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU was similar when injected at E15 than when injected at E14.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	retinal ganglion cell	Present	Regionally restricted		Expression was detected in ipsilateral retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU was sharply lower when injected at E16 than when injected at E15.