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Gene Expression Data
Assay Details
Assay
Reference: J:60642 Gradwohl G, et al., neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas. Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1607-11
Assay type: RNA in situ
MGI Accession ID: MGI:7705870
Gene symbol: Neurog3
Gene name: neurogenin 3
Probe: Math4B genomic1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1a: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: pancreas primordium Strong 1a High levels of expression as early as E9.5.
Specimen 1b: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pancreas primordium Strong 1b Number of expressing cells increases until E15.5. Expressing cells either isolated or grouped in small clusters in pancreatic epithelium at E10.5.
Specimen 1c: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: pancreas Strong Regionally restricted 1c Number of expressing cells increases until E15.5. Expressing cells located within or adjacent to pancreatic ducts.
TS23: exocrine pancreas Absent 1c No expression in exocrine tissue.
Specimen 1d: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: pancreas Present Regionally restricted 1d Number of expressing cells decreases after E15.5. Expressing cells ocated within or adjacent to pancreatic ducts.
Specimen birth not shown: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: pancreas Absent No expressing cells found at birth.
Specimen adult not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: pancreas Absent No expressing cells found in adult.
Specimen 1e: embryonic day 10.5 (more )
Note: Double labeled: blue - Neurog3; brown - Gcg (assay MGI:7705879).
Structure Level Pattern Image Note
TS17: pancreas primordium Strong Regionally restricted 1e Expressing cells either isolated or grouped in small clusters in pancreatic epithelium at E10.5. No co-expression with glucagon.
Specimen 1f: embryonic day 15.5 (more )
Note: Double labeled: blue - Neurog3; brown - Gcg (assay MGI:7705879) and Ins1 (assay MGI:7705881) and Ins2 (assay MGI:7705883).
Structure Level Pattern Image Note
TS23: pancreas Strong Regionally restricted 1f Number of expressing cells increases until E15.5. No co-expression with glucagon or insulin.
TS23: exocrine pancreas Absent 1f No expression in exocrine tissue.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory