Gene Expression Data
Assay Details
Assay
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Reference:
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J:259558
Bhattacharyya S, et al., Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development. Sci Rep. 2016 Feb 17;6:20727
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7703789
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Gene symbol:
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Ehd1
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Gene name:
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EH-domain containing 1
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Antibody:
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anti-EHD1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 633
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Results
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Specimen
3A WT:
embryonic day 9.5
(more )
Specimen
3A WT:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Present |
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3A WT EHD1
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Expressed throughout the cytoplasm of neuroepithelial cells with prominent enrichment underneath the apical surface (facing the lumen).
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Specimen
3A -/-:
(close )
Genetic Background:
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B6.Cg-Ehd1tm1.2Haba
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Mutant Allele(s):
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Ehd1tm1.2Haba/Ehd1tm1.2Haba
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4D:
embryonic day 9.5
(more )
Note: Double labeled: red - Ehd1; green - Tubg1 (assay MGI:7703798) and Tubg2 (assay MGI:7703900).
Specimen
4D:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Ehd1; green - Tubg1 (assay MGI:7703798) and Tubg2 (assay MGI:7703900).
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Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Present |
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4D middle
,
4D right
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Expression was seen in the same compartments of the neural tube as gamma-tubulin, a specific marker of ciliary basal body on the luminal surface of neuroepithelial cells.
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