Neurog2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:46667 Fode C, et al., The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron. 1998 Mar;20(3):483-94
Assay type:	RNA in situ
MGI Accession ID:	MGI:7666577
Gene symbol:	Neurog2
Gene name:	neurogenin 2

Probe:	Math4A cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 11-13 somites not shown: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: geniculate placode	Present			Expression first detected in geniculate placode (VII) in 11-13 somite embryos.

Specimen 1A: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: geniculate placode	Present		1A
TS14: petrosal placode	Present		1A	Expression in petrosal placode (IX) at 18-20 somite stage.

Specimen 1B: embryonic day 9.5 (more

)

Structure	Level	Image	Note
TS15: geniculate placode	Present	1B
TS15: petrosal placode	Present	1B
TS15: nodose placode	Present	1B	Expression in nodose placode (X) at 20-24 somite stage.

Specimen E9.5 not shown: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent
TS15: facio-acoustic preganglion complex	Absent
TS15: glossopharyngeal preganglion	Absent
TS15: vagus preganglion	Absent

Specimen 1C: embryonic day 10.0 (more

)

Structure	Level	Image
TS16: geniculate placode	Present	1C
TS16: nodose placode	Present	1C
TS16: petrosal placode	Present	1C

Specimen E10.25 not shown: embryonic day 10.25 (more

)

Structure	Level	Pattern	Image	Note
TS17: geniculate placode	Absent			Expression disappeared from geniculate placode around E10.25.

Specimen E10.75 not shown: embryonic day 10.75 (more

)

Structure	Level	Pattern	Image	Note
TS17: nodose placode	Absent			Expression disappeared from petrosal and nodose placode around E10.75.
TS17: petrosal placode	Absent			Expression disappeared from petrosal and nodose placode around E10.75.

Specimen 1D: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: petrosal placode	Present	Regionally restricted	1D	At E9.5 (24-28 somites), expression in placodal cells and migrating neural precursors immediately after delamination.
TS15: glossopharyngeal preganglion	Absent		1D	No expression in precursors near or in ganglionic precursors.

Specimen 7C: embryonic day 10.0 (more

)
Note: Double labeled: blue - Neurog2; brown - Phox2a (assay MGI:7666609).

Structure	Level	Pattern	Image	Note
TS16: nodose placode	Present	Regionally restricted	7C	Expression in groups of contiguous placodal cells and migrating precursor cells that have just delaminated.
TS16: vagus ganglion	Strong	Regionally restricted	7C	No expression in precursors near or in ganglionic precursors.

Specimen 7D: embryonic day 10.0 (more

)
Note: Double labeled: blue - Neurog2; brown - Phox2a (assay MGI:7666609).

Structure	Level	Pattern	Image	Note
TS16: nodose placode	Present		7D

Specimen 7E: embryonic day 9.5 (more

)

Structure	Level	Image
TS15: geniculate placode	Present	7E
TS15: nodose placode	Present	7E
TS15: petrosal placode	Present	7E

Specimen 7E': embryonic day 9.5; Phox2a^tm1Jbr/Phox2a^tm1Jbr (more

)

Structure	Level	Image	Note
TS15: geniculate placode	Present	7E'	Levels of expression similar to that of wild type.
TS15: nodose placode	Present	7E'	Levels of expression similar to that of wild type.
TS15: petrosal placode	Present	7E'	Levels of expression similar to that of wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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