Antibody:	anti-NeuroD1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS22: adenohypophysis	corticotroph	Present		1E inset , 1E	Mostly in the nucleus of cells within the developing anterior pituitary. Expressed in a significant number of cells.
TS22: adenohypophysis pars intermedia		Absent		1E

Structure	Cell Type	Level	Pattern	Image	Note
TS24: adenohypophysis	corticotroph	Present		1F	Mostly in the nucleus of cells within the developing anterior pituitary. The number of positive cells was decreased.
TS24: adenohypophysis pars intermedia		Absent		1F

Structure	Cell Type	Level	Pattern	Image	Note
TS26: adenohypophysis	corticotroph	Present		1G	Mostly in the nucleus of cells within the developing anterior pituitary. Expression was much weaker.
TS26: adenohypophysis pars intermedia		Absent		1G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: adenohypophysis	corticotroph	Weak		1H inset , 1H	Predominantly cytoplasmic. Very few positive nuclei remained.
TS28: adenohypophysis pars intermedia		Absent		1H

Structure	Cell Type	Level	Pattern	Image	Note
TS20: adenohypophysis	corticotroph	Present
TS20: pituitary gland		Weak			Low-level expression in other cells (mostly alphaGSU/Cga-positive cells).