Probe:	Gli1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS23: head	Present	Regionally restricted	1C
TS23: eyelid	Strong		1C	Robust expression across all tissues examined.
TS23: hair follicle	Strong		1C	Robust expression across all tissues examined.
TS23: molar	Strong		1C	Robust expression across all tissues examined.
TS23: palatal rugae	Strong		1C	Robust expression across all tissues examined.
TS23: retina	Strong		1C	Robust expression across all tissues examined.

Structure	Level	Pattern	Image	Note
TS23: upper jaw molar	Present	Widespread	2O	Expression throughout epithelial and mesenchymal structures., mirroring that of Ptch1.
TS23: upper jaw molar mesenchyme	Strong	Regionally restricted	2O	Stronger expression in mesenchymal tissues.
TS23: dental sac	Strong		2O	Strongest expression in dental follicle.
TS23: oral region mesenchyme	Present	Regionally restricted	2O	No expression in oral mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: lower jaw molar	Present	Widespread	2P	Expression throughout epithelial and mesenchymal structures., mirroring that of Ptch1.
TS23: lower jaw molar mesenchyme	Strong	Regionally restricted	2P	Stronger expression in mesenchymal tissues.
TS23: dental sac	Strong		2P	Strongest expression in dental follicle.
TS23: oral region mesenchyme	Present	Regionally restricted	2P	No expression in oral mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present	Widespread	2Q	Expression throughout epithelial and mesenchymal structures, largely restricted within border of Hhip expression.
TS23: hair follicle dermal papilla	Strong		2Q	Stronger expression in mesenchymal tissues.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf epithelium	Present		2R	Expression throughout epithelial and mesenchymal structures.
TS23: palatal shelf mesenchyme	Present		2R	Expression throughout epithelial and mesenchymal structures.
TS23: palatal rugae epithelium	Present		2R	Expression in rugae of palatal oral epithelium.
TS23: palatal rugae mesenchyme	Present	Regionally restricted	2R	Expression in adjacent mesenchyme in pattern simlar to that of Ptch1 but more restricted in range and less uniform within mesenchymal component.

Structure	Level	Pattern	Image	Note
TS23: eyelid	Present	Regionally restricted	2S	Expression in both eyelid epithelium and adjacent mesenchyme.
TS23: eyelid epithelium	Strong	Regionally restricted	2S	Stronger expression in basal layer eyelid epithelium.
TS23: eyelid mesenchyme	Present	Regionally restricted	2S	Expression in adjacent mesenchyme of eye tip.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	2T	Expression definitively restricted to neuroblast layer.
TS23: eye anterior segment	Present	Regionally restricted	2T	Expression in stroma of iris.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present		2U	Expression overlapping with that of Ptch1 in neuroblast layer of neural retina
TS23: retina ganglion cell layer	Absent		2U	No expression in differentiated cell layer.
TS23: perioptic mesenchyme	Present	Regionally restricted	2U	Narrow band of expression in scleral condensation of extraocular mesenchyme, marking boundary with retina pigmented epithelium.