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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664545
Gene symbol: Gad2
Gene name: glutamic acid decarboxylase 2
Probe: Gad2_riboprobe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 4bb: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Cell Type Level Pattern Image Note
TS22: mesomere 2 Strong 4bb
TS22: midbrain GABAergic neuron Present 4bb
TS22: inferior colliculus Absent 4bb
Specimen 7q: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: mesomere 2 Present Regionally restricted 7q Expression was detected in the preisthmus and showed a curved boundary with the isthmus, with development of a marked convexity towards the midbrain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory