Gene Expression Data
Assay Details
Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664445
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Gene symbol:
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Sst
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Gene name:
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somatostatin
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Probe:
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Sst_riboprobe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
3q:
embryonic day 11.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3q:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: midbrain |
Present |
Regionally restricted |
3q
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Sst-positive postmitotic cells were observed rostrally in the basal midbrain but were lacking at the caudal sulcal area.
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TS19: midbrain-hindbrain junction |
Present |
Regionally restricted |
3q
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Sst-positive postmitotic cells were observed rostrally in the basal isthmus.
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Specimen
5j:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5j:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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Specimen
8b:
embryonic day 15.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
8b:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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Specimen
8z:
embryonic day 15.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
8z:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mesomere 2 |
Present |
Regionally restricted |
8z
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Selective labeling was observed in the basal or tegmental preisthmus.
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Specimen
11u:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11u:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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Structure |
Level |
Pattern |
Image |
Note |
TS26: mesomere 2 |
Present |
Regionally restricted |
11u
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Expression was detected in the basal tegmental preisthmus.
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Specimen
12c:
postnatal day 4
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
12c:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal day 4
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: midbrain |
Present |
Regionally restricted |
12c
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Expression was detected in an elongated and narrow tissue band at the caudal end of the basal midbrain.
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