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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664444
Gene symbol: Tal2
Gene name: T cell acute lymphocytic leukemia 2
Probe: Tal2_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 3n: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain mantle layer Strong Regionally restricted 3n Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain ventricular layer Strong Regionally restricted 3n Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain Weak Regionally restricted 3n Weak expression was also detected in the alar domain. No staining was observed in the caudal sulcal area.
Specimen 3o: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain mantle layer Strong Regionally restricted 3o Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain ventricular layer Strong Regionally restricted 3o Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain Weak Regionally restricted 3o Weak expression was also detected in the alar domain. No staining was observed in the caudal sulcal area.
Specimen 3p: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain mantle layer Strong Regionally restricted 3p Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain ventricular layer Strong Regionally restricted 3p Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain Weak Regionally restricted 3p Weak expression was also detected in the alar domain. No staining was observed in the caudal sulcal area.
Specimen 5u: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: mesomere 2 Present 5u
TS22: superior colliculus Present 5u
TS22: inferior colliculus Present Regionally restricted 5u A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction Weak Regionally restricted 5u There was only very weak ventricular expression at the isthmus.
Specimen 5v: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: mesomere 2 Present 5v
TS22: superior colliculus Present 5v
TS22: inferior colliculus Present Regionally restricted 5v A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction Weak Regionally restricted 5v There was only very weak ventricular expression at the isthmus.
Specimen 5w: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: mesomere 2 Present 5w
TS22: superior colliculus Present 5w
TS22: inferior colliculus Present Regionally restricted 5w A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction Weak Regionally restricted 5w There was only very weak ventricular expression at the isthmus.
Specimen 5x: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: mesomere 2 Present 5x
TS22: superior colliculus Present 5x
TS22: inferior colliculus Present Regionally restricted 5x A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction Weak Regionally restricted 5x There was only very weak ventricular expression at the isthmus.
Specimen 5y: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: mesomere 2 Present 5y
TS22: superior colliculus Present 5y
TS22: inferior colliculus Present Regionally restricted 5y A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction Weak Regionally restricted 5y There was only very weak ventricular expression at the isthmus.
Specimen 5z: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: mesomere 2 Present 5z
TS22: superior colliculus Present 5z
TS22: inferior colliculus Present Regionally restricted 5z A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction Weak Regionally restricted 5z There was only very weak ventricular expression at the isthmus.
Specimen 11i: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 11i Expression was detected in the ventricular zone of the toral bulges.
TS26: inferior colliculus Absent 11i
Specimen 11j: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 11j Expression was detected in the ventricular zone of the toral bulges.
TS26: inferior colliculus Absent 11j
TS26: mesomere 2 Absent 11j
Specimen 11k: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 11k Expression was detected in the ventricular zone of the toral bulges.
TS26: inferior colliculus Absent 11k
TS26: mesomere 2 Absent 11k
Specimen 11l: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 11l Expression was detected in the ventricular zone of the toral bulges.
TS26: inferior colliculus Absent 11l
TS26: mesomere 2 Absent 11l
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/30/2025
MGI 6.24 		The Jackson Laboratory