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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664433
Gene symbol: Lhx5
Gene name: LIM homeobox protein 5
Probe: Lhx5_riboprobe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 2y: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain mantle layer Present Regionally restricted 2y Expression was detected in the midbrain mantle at an intermediate rostrocaudal level, leaving negative both the rostral pole and the caudal sulcal area.
TS19: mesomere 1 Present 2y
TS19: midbrain-hindbrain junction Present 2y
Specimen 2z: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain mantle layer Present Regionally restricted 2z Expression was detected in the midbrain mantle at an intermediate rostrocaudal level, leaving negative both the rostral pole and the caudal sulcal area.
TS19: mesomere 1 Present 2z
TS19: midbrain-hindbrain junction Present 2z
Specimen 2aa: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain mantle layer Present Regionally restricted 2aa Expression was detected in the midbrain mantle at an intermediate rostrocaudal level, leaving negative both the rostral pole and the caudal sulcal area.
TS19: mesomere 1 Present 2aa
TS19: midbrain-hindbrain junction Present 2aa
Specimen 4j: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 4j
TS22: mesomere 2 Present Regionally restricted 4j Signal was detected in the preisthmic mantle domains (including the caudal sulcal area).
TS22: inferior colliculus Ambiguous 4j The inferior colliculus mantle and ventricular zones were largely negative.
TS22: midbrain-hindbrain junction Absent 4j
Specimen 6h: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6h
TS22: mesomere 2 Present Regionally restricted 6h Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6h A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6h
Specimen 6i: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6i
TS22: mesomere 2 Present Regionally restricted 6i Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6i A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6i
Specimen 6j: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6j
TS22: mesomere 2 Present Regionally restricted 6j Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6j A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6j
Specimen 6k: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6k
TS22: mesomere 2 Present Regionally restricted 6k Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6k A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6k
Specimen 6l: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6l
TS22: mesomere 2 Present Regionally restricted 6l Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6l A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6l
Specimen 6m: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6m
TS22: mesomere 2 Present Regionally restricted 6m Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6m A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6m
Specimen 6n: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: superior colliculus Present 6n
TS22: mesomere 2 Present Regionally restricted 6n Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 6n A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction Present 6n
Specimen 7f: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: mesomere 2 Present 7f
Specimen 8q: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Absent 8q
TS23: superior colliculus Weak Diffuse 8q
TS23: mesomere 2 Present Regionally restricted 8q Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction Absent 8q
Specimen 8r: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Absent 8r
TS23: superior colliculus Weak Diffuse 8r
TS23: mesomere 2 Present Regionally restricted 8r Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction Absent 8r
Specimen 8s: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Absent 8s
TS23: superior colliculus Weak Diffuse 8s
TS23: mesomere 2 Present Regionally restricted 8s Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction Absent 8s
Specimen 8t: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Absent 8t
TS23: superior colliculus Weak Diffuse 8t
TS23: mesomere 2 Present Regionally restricted 8t Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction Absent 8t
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory