Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664417
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Gene symbol:
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Fgf15
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Gene name:
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fibroblast growth factor 15
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Probe:
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Fgf15_riboprobe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
2g:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
2g:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Non-Uniform |
2g
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Expression was weak at the rostral half of the midbrain, but it increases gradentally caudalwards to a maximum just in front of the isthmo-mesencephalic transverse boundary at the expected locus of the m2 mesomere.
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| TS19: mesomere 2 |
Present |
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2g
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Specimen
4z:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
4z:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
Present |
Regionally restricted |
4z
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Signal appeared as a positive transverse line that seemed to separate the negative preisthmic domain from the inferior colliculus domain.
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| TS22: mesomere 2 |
Absent |
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4z
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Specimen
4aa:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
4aa:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
Present |
Regionally restricted |
4aa
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Signal appeared as a positive transverse line that seemed to separate the negative preisthmic domain from the inferior colliculus domain.
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| TS22: mesomere 2 |
Absent |
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4aa
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Analysis Tools