Probe:	Cbln2 probe
Probe preparation:	labelled with P33
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		1C	Higher levels of expression in olfactory bulb, cerebral cortex, and superior and inferior colliculi.
TS28: inferior colliculus	Strong		1C	Higher levels of expression in olfactory bulb, cerebral cortex, and superior and inferior colliculi.
TS28: main olfactory bulb	Strong		1C	Higher levels of expression in olfactory bulb, cerebral cortex, and superior and inferior colliculi.
TS28: superior colliculus	Strong		1C	Higher levels of expression in olfactory bulb, cerebral cortex, and superior and inferior colliculi.
TS28: hypothalamic nucleus	Strong	Regionally restricted	1C	Higher levels of expression in certain thalamic and hypothalamic nuclei.
TS28: thalamic nucleus	Strong	Regionally restricted	1C	Higher levels of expression in certain thalamic and hypothalamic nuclei.
TS28: deep cerebellar nucleus	Absent		1C

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Moderate		2B
TS28: cortical layer III	Moderate		2B
TS28: cortical layer IV	Moderate		2B
TS28: cortical layer VI	Moderate		2B
TS28: cortical layer I	Absent		2B
TS28: cortical layer V	Absent		2B
TS28: anterior dorsal thalamic nucleus	Absent		2B
TS28: anterior ventral thalamic nucleus	Absent		2B
TS28: central medial thalamic nucleus	Strong		2B
TS28: nucleus reuniens	Moderate		2B
TS28: paraventricular thalamic nucleus	Moderate		2B
TS28: paraventricular hypothalamic nucleus	Moderate		2B

Structure	Level	Pattern	Image	Note
TS28: retrosplenial granular cortex	Moderate		2E
TS28: subiculum	Moderate		2E
TS28: CA1 pyramidal cell layer	Absent		2E
TS28: CA3 pyramidal cell layer	Absent		2E
TS28: dentate gyrus granule cell layer	Absent		2E
TS28: parafascicular nucleus	Strong		2E
TS28: dorsal lateral geniculate nucleus	Weak		2E
TS28: arcuate nucleus	Weak		2E

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Present	Regionally restricted	2H	High expression in dorsal nucleus.. Moderate expression in external nuclei. Very weak expression in central nucleus. No expression in nucleus brachium.
TS28: entorhinal cortex	Absent		2H

Structure	Level	Pattern	Image	Note
TS28: solitary tract nucleus	Moderate		2K
TS28: cerebellum granule cell layer	Trace		2K
TS28: cerebellum Purkinje cell layer	Absent		2K
TS28: cerebellum molecular layer	Absent		2K
TS28: deep cerebellar nucleus	Absent		2K

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb	Strong		3B1	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS21: thalamus	Strong		3B1	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Strong		3B2	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS23: main olfactory bulb	Strong		3B2	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS23: thalamus	Strong		3B2	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS23: neocortex	Present	Graded	3B2	Transient expression in gradient-like distribution starting from rostral pole at E15 and spreading caudally.

Structure	Level	Pattern	Image	Note
TS25: inferior colliculus	Strong		3B3	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS25: main olfactory bulb	Strong		3B3	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS25: thalamus	Strong		3B3	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Strong		3B4	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS27: main olfactory bulb	Strong		3B4	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS27: thalamus	Strong		3B4	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS27: trigeminal V spinal sensory nucleus	Strong		3B4	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS27: neocortex layer	Present	Graded	3B4	Rostrocaudal gradient of expression ascribed to different expression levels in cortical plate and differentiating cortical layers.

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Strong		3B5	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: main olfactory bulb	Strong		3B5	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: thalamus	Strong		3B5	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: trigeminal V spinal sensory nucleus	Strong		3B5	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Strong		3B6	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: main olfactory bulb	Strong		3B6	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: thalamus	Strong		3B6	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: trigeminal V spinal sensory nucleus	Strong		3B6	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Strong		3B7	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: main olfactory bulb	Strong		3B7	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: thalamus	Strong		3B7	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.
TS28: trigeminal V spinal sensory nucleus	Strong		3B7	Expression detected as early as E13. Higher levels of expression consistently detected in olfactory bulb, thalamus, inferior colliculus and trigeminal spinal tract nucleus throughout development.

Structure	Level	Pattern	Image	Note
TS27: brain	Present		4F

Structure	Level	Pattern	Image	Note
TS27: brain	Present		4G

Structure	Level	Pattern	Image	Note
TS27: brain	Present		4I

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Graded	4K

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Graded	4L

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Graded	4M

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Graded	4N

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Graded	4O

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Graded	4P

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Widespread	S2B	Widely expressed in olfactory bulb, including glomerular, mitral and granular layers.
TS28: main olfactory bulb glomerular layer	Moderate		S2B
TS28: main olfactory bulb granule cell layer	Moderate		S2B
TS28: main olfactory bulb mitral cell layer	Moderate		S2B

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Moderate		S2E
TS28: cortical layer III	Moderate		S2E
TS28: cortical layer IV	Moderate		S2E
TS28: cortical layer VI	Moderate		S2E
TS28: cortical layer I	Absent		S2E
TS28: cortical layer V	Absent		S2E
TS28: claustrum	Moderate		S2E
TS28: piriform cortex	Weak		S2E
TS28: endopiriform nucleus	Weak		S2E

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Moderate		S2H
TS28: cortical layer III	Moderate		S2H
TS28: cortical layer IV	Moderate		S2H
TS28: cortical layer VI	Moderate		S2H
TS28: cortical layer I	Absent		S2H
TS28: cortical layer V	Absent		S2H
TS28: claustrum	Moderate		S2H
TS28: medial septal nucleus	Weak		S2H

Structure	Level	Pattern	Image	Note
TS28: preoptic area	Strong	Regionally restricted	S2K	High expression in preoptic nucleus.
TS28: hypothalamus	Present	Regionally restricted	S2K	Moderate expression in posterior area and periventricular nucleus. Weak expression in lateral area and dorsal medial nucleus.
TS28: paraventricular hypothalamic nucleus	Moderate		S2K

Structure	Level	Pattern	Image	Note
TS28: retrosplenial granular cortex	Moderate		S3B
TS28: ventral medial thalamic nucleus	Absent		S3B
TS28: ventromedial hypothalamic nucleus	Weak		S3B
TS28: CA1 pyramidal cell layer	Absent		S3B
TS28: CA3 pyramidal cell layer	Absent		S3B
TS28: dentate gyrus granule cell layer	Absent		S3B
TS28: medial habenular nucleus	Moderate		S3B
TS28: lateral habenular nucleus	Strong		S3B
TS28: hypothalamic nucleus	Moderate	Regionally restricted	S3B	Moderate expression in posterior nucleus.

Structure	Level	Pattern	Image	Note
TS28: subiculum	Moderate		S3E
TS28: superior colliculus	Moderate		S3E
TS28: median raphe nucleus	Weak		S3E

Structure	Level	Pattern	Image	Note
TS28: dorsal nucleus of lateral lemniscus	Strong		S3H
TS28: inferior colliculus	Present	Regionally restricted	S3H	High expression in dorsal nucleus. Moderate expression in external nuclei. Very weak expression in central nucleus. No expression in nucleus brachium.
TS28: dorsal cochlear nucleus	Absent		S3H
TS28: ventral cochlear nucleus	Absent		S3H
TS28: trigeminal V spinal sensory nucleus	Trace		S3H
TS28: vestibular nucleus	Trace		S3H
TS28: solitary tract nucleus	Moderate		S3H

Structure	Level	Pattern	Image	Note
TS28: trigeminal V spinal sensory nucleus	Weak		S3K

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		S4A
TS28: dentate gyrus polymorphic layer	Present	Clusters	S4A	Dispersed clusters of expression in polymorphic layer of dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: trigeminal V motor nucleus	Weak
TS28: trigeminal V principal sensory nucleus	Trace
TS28: amygdala	Absent
TS28: caudate-putamen	Absent
TS28: globus pallidus	Absent
TS28: lateral septal nucleus	Absent
TS28: oculomotor nucleus	Absent