Gene Expression Data
Assay Details
Assay
|
Reference:
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J:48211
Sugimoto M, et al., Differential in situ expression of alpha2(XI) collagen mRNA isoforms in the developing mouse. Cell Tissue Res. 1998 May;292(2):325-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7662844
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Gene symbol:
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Col11a2
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Gene name:
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collagen, type XI, alpha 2
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Probe:
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alpha2(XI) exon 6-8 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: notochord |
Absent |
|
|
No significant expression in notochord or other tissues in axial skeleton.
|
TS19: sclerotome |
Absent |
|
|
No significant expression in notochord or other tissues in axial skeleton.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: notochord |
Absent |
|
|
No significant expression in notochord or other tissues in axial skeleton.
|
TS20: sclerotome |
Absent |
|
|
No significant expression in notochord or other tissues in axial skeleton.
|
Specimen
2E:
embryonic day 12.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2F:
embryonic day 13.0
(more )
Specimen
2F:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: limb |
Weak |
Regionally restricted |
2F
|
Weak expression, more promient at periphery of cartilaginous condensations.
|
Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cartilage |
Present |
Regionally restricted |
3C
|
Authors state that expression was not prominent in cartilaginous tissues under low magnification.
|
Specimen
4D:
embryonic day 15.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: periosteum |
Present |
Regionally restricted |
4D
|
Expression in immature periosteum.
|
TS23: radius |
Present |
Regionally restricted |
4D
|
Expression in immature periosteum.
|
Specimen
5C:
embryonic day 15.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: chondrocranium |
Strong |
Regionally restricted |
5C
|
High level of expression in mesenchymal cell layers differentiating into osteoblasts.
|
|