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Gene Expression Data
Assay Details
Assay
Reference: J:171421 Vandunk C, et al., Development, maturation, and necessity of transcription factors in the mouse suprachiasmatic nucleus. J Neurosci. 2011 Apr 27;31(17):6457-67
Assay type: RNA in situ
MGI Accession ID: MGI:7660862
Gene symbol: Vip
Gene name: vasoactive intestinal polypeptide
Probe: Vip probe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2A: postnatal day 15 (more )
Structure Level Pattern Image Note
TS28: suprachiasmatic nucleus Present Regionally restricted 2A VIP Regional-specific expression was detected in the suprachiasmatic nucleus.
Specimen 2B: postnatal day 15 (more )
Note: Triple labeled: blue - Vip; green - Rora (assay MGI:7660842); red - Six3 (assay MGI:7660839).
Structure Level Pattern Image Note
TS28: suprachiasmatic nucleus Present Regionally restricted 2B Overlay , 2B VIP Regional-specific expression was detected in the suprachiasmatic nucleus. There was no overlap with Rora expression.
Specimen not shown 3 of 5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: suprachiasmatic nucleus Present Expression was present in 3 out of 5 animals at E18.5.
Specimen not shown 2 of 5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: suprachiasmatic nucleus Absent Expression was not detected in 2 out of 5 animals at E18.5.
Specimen 5 Rora+/+: postnatal day 15 (more )
Structure Level Pattern Image Note
TS28: suprachiasmatic nucleus Present 5_VIP Rora+/+
Specimen 5 Rorasg/sg: postnatal day 15; Rorasg/Rorasg (more )
Structure Level Pattern Image Note
TS28: suprachiasmatic nucleus Present 5_VIP Rorasg/sg The spatial mRNA expression patterns within the suprachiasmatic nucleus remained unaltered in all P15 sg/sg mutants (four of four) compared with wild-type littermates.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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