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Gene Expression Data
Assay Details
Assay
Reference: J:154534 Martinez G, et al., Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4794-806
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7659035
Gene symbol: Apc
Gene name: APC, WNT signaling pathway regulator
Antibody: Anti-APC
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 3A: embryonic day 13.5 (more )
Note: Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Cell Type Level Pattern Image Note
TS21: lens epithelium lens epithelial cell Present 3A
TS21: lens fiber lens fiber cell Present 3A
TS21: cornea epithelium corneal epithelial cell Strong 3A
Specimen 3B: embryonic day 13.5; Apctm1Tno/Apctm1Tno, Tg(Cryaa-cre)10Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Cell Type Level Pattern Image Note
TS21: lens epithelium lens epithelial cell Absent 3B
TS21: lens fiber lens fiber cell Weak Single cells 3B Only occasional fiber cells still showed expression in the mutant.
TS21: cornea epithelium corneal epithelial cell Strong 3B No change in cornea expression was observed in the mutant.
Specimen not shown WT: embryonic day 15.5 (more )
Note: Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Cell Type Level Pattern Image Note
TS23: lens lens epithelial cell Present Localization patterns of expression in E15.5 wild-type lens was similar to that observed at E13.5.
TS23: lens fiber lens fiber cell Present Localization patterns of expression in E15.5 wild-type lens was similar to that observed at E13.5.
Specimen not shown APC10: embryonic day 15.5; Apctm1Tno/Apctm1Tno, Tg(Cryaa-cre)10Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Cell Type Level Pattern Image Note
TS23: lens epithelium lens epithelial cell Absent Localization patterns of expression in E15.5 APC10 lens was similar to that observed at E13.5.
TS23: lens fiber lens fiber cell Weak Single cells Localization patterns of expression in E15.5 APC10 lens was similar to that observed at E13.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory