Gene Expression Data
Assay Details
Assay
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Reference:
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J:62806
Gad JM, et al., The spatial and temporal expression patterns of netrin receptors, DCC and neogenin, in the developing mouse retina. Exp Eye Res. 2000 Jun;70(6):711-22
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7647258
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Gene symbol:
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Dcc
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Gene name:
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DCC netrin 1 receptor
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Antibody:
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2744
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
5A:
embryonic day 15.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina nerve fiber layer |
retinal ganglion cell |
Strong |
Regionally restricted |
5A
,
5B
|
Expression was detected on the retinal ganglion cell axons within the nerve fiber layer.
|
TS23: retina nuclear layer |
Mueller cell |
Present |
Regionally restricted |
5B
,
5A
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Expression was detected in elongated DCC-positive processes extending to the outer limiting membrane of the outer nuclear layer.
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TS23: retina nuclear layer |
|
Present |
Regionally restricted |
5B
,
5A
|
At the outer edge of the outer nuclear layer, cell bodies expressing significant levels of DCC protein were distributed sparsely. Overall, the level of DCC protein detected in the E15.5 outer nuclear layer was low.
|
Specimen
5C:
embryonic day 16.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5E:
embryonic day 18.5
(more )
Specimen
5E:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6A:
embryonic day 15.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: optic disc |
retinal ganglion cell |
Strong |
|
6A
|
Strong expression was detected on the retinal ganglion cell axons within the optic disc.
|
Specimen
6B:
embryonic day 16.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: optic disc |
retinal ganglion cell |
Present |
Regionally restricted |
6B
|
Expression was detected on a subset of the retinal ganglion cell axons within the optic disc at E16.5.
|
Specimen
6C:
embryonic day 18.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|