Gene Expression Data
Assay Details
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Assay
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Reference:
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J:243644
Gobius I, et al., Astroglial-mediated remodeling of the interhemispheric midline during telencephalic development is exclusive to eutherian mammals. Neural Dev. 2017 May 30;12(1):9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7647235
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Gene symbol:
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Slc1a3
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Gene name:
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solute carrier family 1 (glial high affinity glutamate transporter), member 3
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Antibody:
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Anti-EAAT1 (ab416)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 647
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Results
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Specimen
3a:
embryonic day 15.5
(more  )
Note: Either CD1 or C57BL/6 mice were used. Double labeled: red - Slc1a3; green - Gfap (assay MGI:7647236).
Specimen
3a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Either CD1 or C57BL/6 mice were used. Double labeled: red - Slc1a3; green - Gfap (assay MGI:7647236).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: forebrain |
forebrain radial glial cell |
Present |
Regionally restricted |
3a Glast/Gfap
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In mice, radial midline zipper glial cells cells were Slc1a3-positive/Gfap-negative bipolar cells that have an apical attachment to the surface of the third ventricle (3V) and a pial attachment to the interhemispheric fissure.
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Specimen
3c:
embryonic day 16.5
(more )
Note: Either CD1 or C57BL/6 mice were used. Double labeled: red - Slc1a3; green - Gfap (assay MGI:7647236).
Specimen
3c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Either CD1 or C57BL/6 mice were used. Double labeled: red - Slc1a3; green - Gfap (assay MGI:7647236).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
forebrain radial glial cell |
Present |
Regionally restricted |
3c Glast/Gfap
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Expression was detected in radial midline zipper glial cells.
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Specimen
3e:
embryonic day 17.5
(more )
Note: Either CD1 or C57BL/6 mice were used. Double labeled: red - Slc1a3; green - Gfap (assay MGI:7647236).
Specimen
3e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Either CD1 or C57BL/6 mice were used. Double labeled: red - Slc1a3; green - Gfap (assay MGI:7647236).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: forebrain |
forebrain radial glial cell |
Present |
Regionally restricted |
3e Glast/Gfap
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Expression was detected in radial midline zipper glial cells.
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Analysis Tools