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Gene Expression Data
Assay Details
Assay
Reference: J:190449 Korostowski L, et al., The Kcnq1ot1 long non-coding RNA affects chromatin conformation and expression of Kcnq1, but does not regulate its imprinting in the developing heart. PLoS Genet. 2012 Sep;8(9):e1002956
Assay type: RT-PCR
MGI Accession ID: MGI:7647174
Gene symbol: Kcnq1ot1
Gene name: KCNQ1 overlapping transcript 1
Probe: Kcnq1ot1-pF, Kcnq1ot1-pR AS-RT/2kb
Assay notes: Imprinting assay - allele-specific RT-PCR. Abbreviations: M, maternal; P, paternal; N, non-digested; D, Digested; B, C57BL/6J; C, B6(CAST7).
Results
Image: S1A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
CxB Heart E10.5 N E10.5 TS17: heart Not Applicable Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x C57BL/6J)F1 Not Specified
CxB Heart E10.5 D E10.5 TS17: heart Present Weak Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x C57BL/6J)F1 Not Specified Digested with StuI.
CxB Heart E12.5 N E12.5 TS20: heart Not Applicable Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x C57BL/6J)F1 Not Specified
CxB Heart E12.5 D E12.5 TS20: heart Present Weak Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x C57BL/6J)F1 Not Specified Digested with StuI.
CxB Heart E16.5 N E16.5 TS24: heart Not Applicable Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x C57BL/6J)F1 Not Specified
CxB Heart E16.5 D E16.5 TS24: heart Present Weak Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x C57BL/6J)F1 Not Specified Digested with StuI.
Notes:
(a) In the digested (D) lanes, this represents the B6(CAST7) band. Paternal.
(b) In the digested (D) lanes, this represents the two C57BL/6J bands. Maternal.
(c) Non-digested (N) lanes.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory