Kcnq1ot1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:190449 Korostowski L, et al., The Kcnq1ot1 long non-coding RNA affects chromatin conformation and expression of Kcnq1, but does not regulate its imprinting in the developing heart. PLoS Genet. 2012 Sep;8(9):e1002956
Assay type:	RT-PCR
MGI Accession ID:	MGI:7647016
Gene symbol:	Kcnq1ot1
Gene name:	KCNQ1 overlapping transcript 1

Probe:	Kcnq1ot1-pF, Kcnq1ot1-pR AS-RT/2kb
Assay notes:	Imprinting assay - allele-specific RT-PCR. Abbreviations: M, maternal; P, paternal; N, non-digested; D, Digested; B, C57BL/6J; C, B6(CAST7).

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
BxC Heart E10.5 N	E10.5	TS17: heart	Not Applicable	Not Applicable	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified
BxC Heart E10.5 D	E10.5	TS17: heart	Weak	Present	Not Applicable	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified	Digested with StuI.
BxC Heart E12.5 N	E12.5	TS20: heart	Not Applicable	Not Applicable	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified
BxC Heart E12.5 D	E12.5	TS20: heart	Present	Present	Not Applicable	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified	Digested with StuI.
BxC Heart E16.5 N	E16.5	TS24: heart	Not Applicable	Not Applicable	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified
BxC Heart E16.5 D	E16.5	TS24: heart	Present	Present	Not Applicable	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified	Digested with StuI.
BxC Heart P2 N	P2	TS27: heart	Not Applicable	Not Applicable	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified
BxC Heart P2 D	P2	TS27: heart	Present	Present	Not Applicable	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified	Digested with StuI.
BxC P2 Brain N	P2	TS27: brain	Not Applicable	Not Applicable	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified
BxC P2 Brain D	P2	TS27: brain	Absent	Present	Not Applicable	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified	Digested with StuI.
BxC P2 Liver N	P2	TS27: liver	Not Applicable	Not Applicable	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified
BxC P2 Liver D	P2	TS27: liver	Absent	Present	Not Applicable	Not Specified; total RNA	(C57BL/6J x C57BL/6J-Chr 7^CAST/EiJ/Msb)F1		Not Specified	Digested with StuI.

Notes:
(a) In the digested (D) lanes, this represents the C57BL/6J band. Maternal Kcnq1ot1 becomes activated as development progresses in the heart. However, in brain and liver, Kcnq1ot1 remains monoallelic.
(b) In the digested (D) lanes, this represents the two B6(CAST7) bands. Paternal.
(c) Non-digested (N) lanes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 01/06/2026 MGI 6.24