Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63176
Raballo R, et al., Basic fibroblast growth factor (Fgf2) is necessary for cell proliferation and neurogenesis in the developing cerebral cortex. J Neurosci. 2000 Jul 1;20(13):5012-23
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7639659
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Gene symbol:
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Rbfox3
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Gene name:
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RNA binding protein, fox-1 homolog (C. elegans) 3
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Antibody:
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anti-NeuN
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4a:
postnatal day 1
(more  )
Specimen
4a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: frontal cortex |
cerebral cortex neuron |
Present |
Regionally restricted |
4a
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Expression is in neurons in all layers. There is nuclear staining in large, pyramidal-shaped cells in layer 5/6.
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Specimen
4b:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Fgf2tm1Doe/Fgf2tm1Doe
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: frontal cortex |
cerebral cortex neuron |
Present |
Regionally restricted |
4b
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Expression in neurons in the upper cortical layers is similar to wild-type. The staining in large, pyramidal-shaped cells in layer 5/6 is absent in this mutant.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Fgf2tm1Doe/Fgf2tm1Doe
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: parietal cortex |
cerebral cortex neuron |
Present |
Regionally restricted |
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Expression in neurons. The staining in large, pyramidal-shaped cells is absent in this mutant.
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Analysis Tools
