Antibody:	Anti-AQP3
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens epithelium		Present	Regionally restricted	3F , 3B	Cytoplasmic expression in epithelium. Expression primarily contained in cytoplasmic membrane vesicles.
TS28: lens peripheral outer cortex	lens fiber cell	Present	Regionally restricted	3F , 3B	Cytoplasmic expression in fiber cells of peripheral outer cortex. Expression primarily contained in cytoplasmic membrane vesicles.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens deep outer cortex	lens fiber cell	Present	Regionally restricted	3G , 3C	Cytoplasmic localization of expression persisted in differentiating fiber cells of deeper outer cortex.
TS28: lens cortex	lens fiber cell	Weak	Regionally restricted	3G , 3C	Decrease in expression in fiber cells localized in transition zone between outer cortex and inner cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens inner cortex	secondary lens fiber	Present	Regionally restricted	3H , 3D	Expression in mature fiber cells of inner cortex was predominantly associated with plasma membranes.

Structure	Level	Pattern	Image	Note
TS28: lens core	Present	Regionally restricted	3E , 3I	Expression in core predominantly associated with plasma membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: lens vesicle	primary lens fiber	Absent		4D , 4B	Expression absent in elongating primary fibers cells of lens vesicle at E10.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: lens vesicle	lens fiber cell	Present	Patchy	4C , 4E	Expression strongly associated with membranes in distinct patches in fiber cells throughout lens vesicle.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: lens epithelium		Present	Regionally restricted	5B	Cytoplasmic expression in epithelium.
TS24: lens peripheral outer cortex	secondary lens fiber	Present	Regionally restricted	5B	Expression predominantly cytoplasmic in secondary fiber cells in peripheral outer cortex.

Structure	Level	Pattern	Image	Note
TS24: lens inner cortex	Present	Regionally restricted	5C	Expression remained cytoplasmic in inner cortex.

Structure	Level	Pattern	Image	Note
TS24: lens core	Present	Regionally restricted	5D	Cytoplasmic expression in core.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: lens epithelium		Present	Regionally restricted	5F	Cytoplasmic expression in epithelium.
TS27: lens peripheral outer cortex	secondary lens fiber	Present	Regionally restricted	5F	Cytoplasmic expression in differentiated secondary fiber cells of peripheral outer cortex.

Structure	Level	Pattern	Image	Note
TS27: lens inner cortex	Present	Regionally restricted	5G	Cytoplasmic expression persisted in inner cortex.

Structure	Level	Pattern	Image	Note
TS27: lens core	Present	Regionally restricted	5H	Cytoplasmic expression in core.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present	Regionally restricted	6B	Cytoplasmic expression in epithelium.
TS28: lens peripheral outer cortex	Present	Regionally restricted	6B	Cytoplasmic expression in peripheral outer cortex.

Structure	Level	Pattern	Image	Note
TS28: lens deep outer cortex	Present	Regionally restricted	6C	Cytoplasmic expression in deeper outer cortex.

Structure	Level	Pattern	Image	Note
TS28: lens inner cortex	Present	Regionally restricted	6D	Cytoplasmic expression in inner cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens core	primary lens fiber	Present	Regionally restricted	6E	Expression in central primary fibers cells strongly assocated with membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens epithelium		Present	Regionally restricted	6G	Strongly cytoplasmic expression in epithelium.
TS28: lens peripheral outer cortex	secondary lens fiber	Present	Regionally restricted	6G	Strongly cytoplasmic expression in secondary fiber cells of peripheral outer cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens deep outer cortex	secondary lens fiber	Present	Regionally restricted	6H	Strongly cytoplasmic expression in secondary fiber cells of deeper outer cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens inner cortex	secondary lens fiber	Present	Regionally restricted	6I	Expression in mature secondary fiber cells are strongly associated with membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens core	primary lens fiber	Present	Regionally restricted	6J	Expression remained membranous in primary fiber cells of core.

Structure	Level	Pattern	Image	Note
TS28: lens peripheral outer cortex	Present	Regionally restricted	7B	Cytoplasmic expression maintained in peripheral outer cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens deep outer cortex	lens fiber cell	Present	Regionally restricted	7C	Cytoplasmic expression remained in differentiating fiber cells in deeper outer cortex.

Structure	Level	Pattern	Image	Note
TS28: lens inner cortex	Present	Regionally restricted	7D	Expression strongly associated with membranes in inner cortex.

Structure	Level	Pattern	Image	Note
TS28: lens	Present		7E

Structure	Level	Pattern	Image	Note
TS28: lens core	Present	Regionally restricted	7F	Membranous expression in core.